流式細胞儀（flow cytometry）為一臨床檢驗及生物學研究上相當重要的工具，除了可根據檢體（通常為細胞）之光學性質及其所帶的螢光種類進行細胞種類分佈的分析外，更可根據每一細胞的分析結果進行收集，以針對特定細胞群進行更進一步之研究。然而由於流式細胞儀體積龐大、造價昂貴、僅能同時偵測有限種類的光學標的，且在分選時至多只能同時收集四種不同的細胞，因此在使用上仍有不便。抗體晶片將多種抗體以陣列方式排列在基材上，可同時針對大量（數百種）抗原進行快速地分析，除了操作簡單外，還具有分析成本低廉之優點。然而，目前的抗體晶片只具有分析，而不具有標的細胞收集之功能，且目前也無研究探討收集抗體晶片上之標的細胞的可能機制。
為了使目前抗體晶片具有釋放標的細胞之功能，本研究測試了三種可能的釋放機制。1.利用酵素分解抗體結構--本研究以螢光標定之蛋白進行釋放測試，研究結果顯示胰蛋白酶作用30分鐘之釋放效率約為60％，且可成功釋放抗體晶片上之Jurkat細胞。2.利用溫度敏感而使流變性質改變之水膠作為釋放基材--由於高濃度之F127對細胞有毒殺作用，且F127之濃度及環境鹽類濃度易受影響，因此並非是個良好的釋放方法。3.利用表面接枝NIPAM之PDMS於溫度變化產生之親-疏水特性變化進行捕捉及釋放—本研究由基材之溫度敏感膨潤現象及水接觸角變化推測光接枝NIPAM於PDMS基材上為可行且成功的。另外，本研究也藉由奈米聚苯乙烯球之捕捉釋放實驗及小牛血清白蛋白之吸附測試得知表面修飾NIPAM之PDMS具有抗非特異性吸附之特性，且於37℃時具有捕捉疏水性分子之效果。
本研究結果對於釋放抗體晶片上之標的細胞提供了一些解決方向。未來期望本研究能結合微流體系統之設計，製造一同時具有多標的分析及收集功能之細胞篩選晶片。

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