摘要
生物農藥已然成為國家未來優先發展之生技產業之一，而最廣為農用的便是蘇力菌製劑。本研究使用的菌株，蘇力菌達姆斯塔丁斯亞種（Bacillus thuringiensis subsp. darmstadiensis, HD-199）所生產之蘇力菌素，因具熱穩定性和廣譜殺蟲作用，適可彌補δ-內毒素之不足，增加殺蟲能力，故有商業化潛力。產品要商業化，便要透過規模放大的技術，而規模放大之前充分地瞭解系統，有助於成功的放大。

本研究在於利用小規模醱酵槽來探討放大規模時所必需注意的混合問題。在混合良好的實驗室規模醱酵槽，運用pH block pulse及pH periodic variation的技巧來營造混合不佳時的擾動情形，以研究在大規模醱酵槽中，若混合不佳且由於控制pH值時添加酸鹼所造成的影響。結果發現，在生長停滯期，酸鹼值的擾動對系統不會造成影響，但在對數生長期中的擾動則同時影響到菌體生長及產物的生成，且對菌體影響甚於產物。另外，本研究同時運用攪拌速率及通氣量的block pulse及step change 之技巧以探討氧氣質傳以及剪應力在不同時期所造成的影響。結果發現，在對數生長期時氧氣質傳扮演重要角色，供氧不足造成菌體生長受限，產物也隨之降低；在生長停滯期降低剪應力有助於產物的生成。

Abstract
Bioinsecticide is one of the important products of bioindustries. In Taiwan, the government is promoting. The bioinsecticides derived from Bacillus thuringiensis have been globally used. Thuringiensin which is heat-stable with broadly insecticidal activity is produced by Bacillus thuringiensis subsp. darmstadiensis, HD-199, in this study. Thuringiensin can improve the insecticidal activity of the traditional biocontrol agent derived fromδ-endotoxin. So it have highly commercial potential. Scale-up of the fermentor makes the commercialization come true. Understanding the dynamics of the fermentation system can contribute a satisfactory scale-up.

In this study, mixing, which is an important factor in large-scale fermentor, is investigated in the laboratory scale fermentor. In the laboratory scale fermentor, which is well mixed, the technique of pH block pulse and pH periodic variation is employed. The change of pH value simulates poor mixing in a large-scale fermentor. We found that the variations of pH did not have effects on the production of product during the stationary phase, but significantly affected both the growth of microbes and the production of thuringiensin during the exponential phase.

Similarly, we introduced the block pulse and step change of agitation and aeration to investigate the problem of oxygen transfer and the effect of shear stress. The oxygen transfer played an important role during the exponential phase. It limited the growth of the microbes and reduced the production of thuringiensin, if the oxygen was not sufficiently provided. The yield could be improved by reducing the agitation rate during the stationary phase.

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