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研究生: 溫庭均
Wen, Ting-Chun
論文名稱: 保存魚類標本之自然體色:以金魚為例
Preserving natural color of fish specimens : Taking goldfish (Carassius auratus) as an example
指導教授: 李家維
口試委員: 邵廣昭
莊永仁
學位類別: 碩士
Master
系所名稱: 生命科學暨醫學院 - 分子與細胞生物研究所
Institute of Molecular and Cellular Biology
論文出版年: 2013
畢業學年度: 101
語文別: 中文
論文頁數: 95
中文關鍵詞: 魚類標本金魚
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  • 有關於魚體標本的保存,過去以來主要以福馬林或70%乙醇來做保存, 此方法雖然可保存魚體的型態,但無法維持魚體的自然體色,本研究以橘色金魚(Carassius auratus)作為實驗對象,並成功的保存金魚之體色。金魚呈色的主要色素為類胡蘿蔔素,此色素含有長鏈的共軛雙鍵,因此容易受到環境等因素的影響,像是,光照、溫度、酸鹼、氧化,因此要維持金魚的色澤,必須使類胡蘿蔔素處於穩定的狀態。本實驗以抗氧化劑(ascorbic acid, AA、erythorbic acid, EA、sodium sulfite, SS、sodium pyrosulfite, SP、sodium bisulfate, SB)作為穩定類胡蘿蔔素的主要配方,並以CIE L*a*b色彩空間作魚體顏色變化的判斷。色度分析結果顯示每種抗氧化劑對金魚都有一定能力的保色效果,其中又以AA效果最佳,此外將AA混合加入SP以後,發現保色效果更佳。由結果驗證添加抗氧化劑作可以有效的保存金魚的體色,並可進一步發展為保存其它魚類標本的潛力。


    Formalin and 70% ethanol are used to preserve fish specimens for long time, since they are effective in preserving morphology; however, this method is not able to preserve the natural color. In this research, we provide a new approach to preserve the natural color of goldfish (Carassius auratus). Goldfish has bright orange color, which are mainly composed of carotenoid. This particle has long chain of conjugated double bonds thus is very unstable and susceptible to environmental factors such as light, temperature, pH and oxidation. Therefore, to stabilize carotenoid in fish body is the key step of preserving its nature color. We attempt to stabilize carotenoid by various antioxidants (ascorbic acid, erythorbic acid, sodium sulfite, sodium pyrosulfite, sodium bisulfite) and compare their abilities of preserving color. The results show that each antioxidant has different abilities to preserve color of goldfish, and the ascorbic acid is the best in our experiments. Additionally, we observe that the color preserved by mixing ascorbic acid and sodium pyrosulfite is more closely to the original one. These results suggest that antioxidants can effectively preserve the color of goldfish and also have the potential application to other fish specimens.

    目錄.................................................1 一、前言.................................................5 二、文獻探討.............................................6 1. 金魚簡介..........................................6 2. 魚體呈色系統......................................7 2.1 魚類色素細胞.................................7 2.1.1 黑色素細胞.............................7 2.1.2 紅色素細胞與黃色素細胞.................8 2.1.3 藍色素細胞.............................8 2.1.4 彩虹色素細胞與白色素細胞................9 2.2 魚類體色變化 ...............................10 3. 金魚呈色之色素-類胡蘿蔔素........................11 3.1 金魚色素....................................11 3.2 類胡蘿蔔素..................................13 3.3 影響類胡蘿蔔素穩定的因子....................16 3.3.1 氧氣..................................17 3.3.2 光照..................................18 3.3.3 溫度..................................18 3.3.4 酸鹼..................................18 3.3.5 其它 ................................19 4. 標本製作與保存...................................20 4.1 魚類標本保存方法探討.........................20 4.2 標本固定.....................................21 5.色彩系統..........................................23 三、實驗方法與材料......................................26 1. 實驗流程.........................................26 2. 實驗材料.........................................27 2.1 金魚來源....................................27 2.2 飼養環境與篩選..............................27 3. 金魚浸液標本製作.................................28 3.1 金魚樣本浸泡前處理..........................28 3.2 金魚保色標本浸泡液體配製....................28 4. 金魚樣本色度分析.................................29 4.1 色度分析方法.................................29 4.2 色度值與顏色差異值...........................30 4.3 保存條件色度分析.............................30 4.3.1 前處理分析分析.........................30 4.3.2 光照影響分析...........................31 4.3.3 酸鹼影響分析...........................32 4.3.4 溫度影響分析...........................32 4.3.5 抗氧化劑分析..........................32 4.3.6 混合抗氧化劑分析.......................33 5. 類胡蘿蔔素萃取與定量.............................34 5.1 類胡蘿蔔素萃取.............................34 5.2 類胡蘿蔔素定量..............................34 6. 標本浸泡液體抗氧化分析...........................35 6.1 DPPH自由基清除能力..........................35 6.2 DPPH自由基清除時效..........................36 7. 金魚浸泡保存液之色素細胞顯微觀察.................36 四、 實驗結果...........................................37 1.金魚保存條件色度分析與外觀評比....................37 1.1 固定前處理結果...............................37 1.2 光照結果.....................................39 1.3 酸鹼結果.....................................42 1.4 溫度結果.....................................45 1.5 抗氧化劑結果.................................47 1.6 混合抗氧化劑分析結果.........................56 2.金魚浸泡保存液分析................................59 2.1 金魚浸泡保存液之類胡蘿蔔素定量...............59 2.2 保存液體抗氧化效能分析.......................61 五、討論................................................62 1.標本固定探討......................................62 2.保存條件探討......................................63 3.保存液之保色原因探討..............................64 4.抗壞血酸與亞硫酸鹽類作用探討......................65 5.顏色變化與型態改變探討............................66 6.其它色素保存與方法探討............................68 7.魚類保色標本建議做法..............................69 8.未來工作與發展....................................70 六、結論................................................71 七、參考文獻............................................72 八、附錄................................................79 1.附錄一............................................79 2.附錄一............................................86 3.附錄三............................................93

    Alger, M. S. M. (1997). Polymer science dictionary. Chapman and Hall, London.
    Bagnara, J. T. (1966). Cytology and cytophysiology of non-melanophore pigment
    cells. International Review of Cytology 20: 173-176.
    Bagnara, J. T. and Hadley, M. E. (1973). Chromatophores and color change.
    Prentice-hall, Englewood Cliffs, New Jersey.
    Bagnara, J. T., Taylor, J. D. and Hadley, M. E. (1968). The dermal chromatophore unit. The Journal of Cell Biology 38: 67-79.
    Balon, E. K. (2004). About the oldest domesticates among fishes. Journal of Fish Biology 65(Supplement A): 1-27.
    Bendich, A. and Olson, J. A. (1989). Biological actions of carotenoids. Federation of American Societies for Experimental Biology 3: 1927-1932.
    Britton, G. (1995). Structure and properties of carotenoids in relation to function. Federation of American Societies for Experimental Biology 9: 1551-1558.
    Chakraborty, A., Sakai, M. and Iwatsuki, Y. (2006). Museum fish specimens and molecular taxonomy: A comparative study on DNA extraction protocols and preservation techniques. Journal of Applied Ichthyology 22(2): 160-166.
    Chen, B. H., Chen, T. M. and Chien, J. T. (1994). Kinetic model for studying the isomerization of α-and β-Carotene during Heating and Illumination. Journal of Agricultural and Food Chemistry 42(11): 2391-2397.
    Chen, H. M. and Meyers, S. P. (1982). Effect of antioxidants on stability of astaxanthin pigment in crawfish waste and oil extract. Journal of Agricultural and Food Chemistry 30(3): 469-473.
    Choi, M. H., Kim, G. H. and Lee, H. S. (2002). Effects of ascorbic acid retention on juice color and pigment stability in blood orange (Citrus sinensis) juice during refrigerated storage. 35(8): 753-759.
    CIE (1986). Colorimetry. CIE Pub. 15.2, 2nd ed, Commission International de L'éclairage. 29-30.
    Distefano, R. J., Roell, M. J., Wanger, B. A. and Decoske, J. J. (1994). Relative performances of four preservatives on fish and crayfish. Transactions of the American Fisheries Society 123(5): 817-823.
    Eklund, P. C., Långvik, O. K., Wärnå, J. P., Salmi, T. O., M., W. S. and Sjöholm, R. E. (2005). Chemical studies on antioxidant mechanisms and free radical scavenging properties of lignans. Organic and Biomolecular Chemistry 3: 3336-3347.
    Florian, M. J. (1990). The effects of freezing and freeze-drying on natural history specimens. Collection forum 6(2): 45-52.
    Fox, C. H., Johnson, F. B., Whiting, J. and Roller, P. P. (1985). Formaldehyde fixation. The Journal of Histochemistry and Cytochemistry 33: 845-853.
    Fox, D. L.(1976). Animal biochromes and structural colors: physical, chemical, distributional and physiological features of colored bodies in the animal world. Berkeley, University of California.
    Fox, D. L. (1936). Further studies of the carotenoids of two pacific marine fishes, Fundulus parvipinnis and Hypsypops rubicunda, and of a marine annelid, Thoracophelia Sp. Proceedings of the National Academy of Sciences 22: 50-54.
    Frankel, E. M. (1985). Chemistry of free radical and singlet oxidation of lipids. Progress in Lipid Research 23: 197-221.
    Fujii, R. (2000). The Regulation of motile activity in fish chromatophores. Pigment Cell Research 13(5): 300-319.
    Gerrick, D. J. (1968). A comparative study of antioxidants in color preservation of fish. The Ohio Journal of Science 68(4): 239-240.
    Goodwin, T. W. (1984). The biochemistry of the carotenoids volume II animals. Chapman and Hall, Landon.
    Hagens, G. V., Tiedemann, K. and Kriz, W. (1987). The current potential of plastination. Anatomy and Embryology 175(4): 411-421.
    Hamazaki, S., Koshiba, M., Habuchi, T., Takahashi, R. and Sugiyama, T. (1993). The effect of formalin fixation on restriction endonuclease: digestion of DNA and PCR amplification. Pathology-Research and Practice 189(5): 553-557.
    Hata, M. (1971). Carotenoi pigments in goldfish (Carassius auratus): I. Composition and distribution of carotenoids. International Journal of Biochemistry 2(7): 11-19.
    Hawkes, J. W. (1974). The structure of fish skin. Cell and Tissue Research 149(2): 159-172.
    Henry, R. W., Janick, L. and Henry, C. (1997). Specimen preparation for silicone plastination. Journal of the International Society For Plastination 12: 13-17.
    Hill, B. H., Roger, T. H. and Vorhagen, F. W. (1997). Comparative analysis of the quantization of color spaces on the basis of the CIELAB color-difference formula. ACM Transactions on Graphics 16: 109-154.
    Hopwood, D. (1985). Cell and tissue fixation, 1972-1982. The Histochemical Journal 17(4): 389-442.
    Hsieh, R. J. and Kinsella, J. E. (1989). Oxidation of polyunsaturated fatty acids: Mechanisms, products, and inhibition with emphasis on fish. Advances in Food and Nutrition Research 33: 233-241.
    Jain, A. K. (1989). Fundamentals of digital image processing. Prentice-Hall, Inc, Upper Saddle River, NJ, USA.
    Katayama, T., Yokoyama, H. and Chichester, C. O. (1970). The biosynthesis of astaxanthin. I. The structure of α-doradexanthin and β-doradexanthin. International Journal of Biochemistry 1(4): 438-444.
    Komiyama, T., Kobayashi, H., Tateno, Y., Inoko, H., Gojobori, T. and Ikeo, K. (2009). An evolutionary origin and selection process of goldfish. Gene 430(1-2): 5-11.
    Koshiba, M., Ogawa, K., Hamazaki, S., Sugiyama, T., Ogawa, O. and Kitajima, T. (1993). The effect of formalin fixation on DNA and the extraction of high-molecular-weight DNA from fixed and embedded tissues. Pathology-Research and Practice 189(1): 66-72.
    Kuehni, R. G. (1976). Color-tolerance data and the tentative CIE 1976 L*a*b* formula. Journal of the Optical Society of America 66(5): 494-500.
    Macdonald, S. J. (1997). The shrinkage of sockeye salmon fry fixed in 10% formalin and preserved in 37.5% isopropano. Fisheries and Oceans Canada, Sciences Branch, Pacific Region, West Vancouver Laboratory, West Vancouver, BC.
    Maeda, E. E. and Mussa, D. M. D. N. (1986). The stability of vitamin C (L-ascorbic acid) in bottled and canned orange juice. Food Chemistry 22(1): 51-58.
    Masamba, K. G. and Mndalira, K. (2013). Vitamin C stability in pineapple, guava and baobab juices under different storage conditions using different levels of sodium benzoate and metabisulphite. Journal of Biotechnology 12(2): 186-191.
    Matsumoto, J. (1965). Studies on the fine structure and cytochemical properties of erythrophores in swordtail, Xiphophorus helleri, with special reference to their pigment granules (pterinosomes). The Journal of cell biology 27: 493-504.
    Mortensen, A. and Skibsted, L. H. (2000). Kinetics and Mechanism of the Primary Steps of Degradation of Carotenoids by Acid in Homogeneous Solution. Journal of Agricultural and Food Chemistry 48(2): 279-286.
    Nordlund, J. J., Boissy, R. E., Hearing, V. J., King, R. A. and Ortonne, J. P. (1998).The
    pigmentary system: physiology and pathophysiology Oxford University Press,
    USA.
    Oliver, J. and Palou, A. (2000). Chromatographic determination of carotenoids in
    foods. Journal of Chromatography A 881(1-2): 543-555.
    Pan, C. H., Chien, Y. H. and Cheng, J. H. (2001). Effects of light regime, algae in the water, and dietary astaxanthin on pigmentation, growth, and survival of black tiger prawn Penaeus monodon post-larvae. Zoological Studies 40(4): 371-382.
    Paripatananont, T., Tangtrongpairoj, J., Sailasuta, A. and Chansue, N. (1999). Effect of Astaxanthin on the Pigmentation of Goldfish Carassius auratus. Journal of the World Aquaculture Society 30(4): 454-460.
    Parker, R. R. (1963). Effects of formalin on length and weight of fishes. Journal of the Fisheries Board of Canada 20(6): 1441-1455.
    Porta, G. (1992). Melanins and melanogenesis. Academic Press New York.
    Qian, C., Decker, E. A., Xiao, H. and McClements, D. J. (2012). Physical and chemical stability of β-carotene-enriched nanoemulsions: Influence of pH, ionic strength, temperature, and emulsifier type. Food Chemistry 132: 1221-1229.
    Rodriguez-Amaya, D. B. and Kimura, M. (2004). HarvestPlus handbook for carotenoid analysis. International Food Policy Research Institute.
    Shimada, K., Fujikawa, K., Yahara, K. and Nakamura, T. (1992). Antioxidative properties of xanthan on the autoxidation of soybean oil in cyclodextrin emulsion. Journal of Agricultural and Food Chemistry 40(6): 945-948.
    Simmon (2002). Directions for preserving small herpetological specimens in formalin. International Society for the History and Bibliography of Herpetology 3(2): 6-7.
    Smartt, J. (2001). Goldfish varieties and genetics. Fishing News Books, United
    Kingdom.
    Smith, C. W. and Hamilton, D. L. (1998). Consolidation of formalin treated marine crustacean specimens using silicone oils, Archaeological Preservation Research Laboratory,Texas A&M University, College Station.
    Smith, T. and Guild, J. (1931). The C.I.E. colorimetric standards and their use. Transactions of the Optical Society 33: 73-134.
    Taylor, J. D. (1969). The effects of intermedin on the ultrastructure of amphibian iridophores. General and Comparative Endocrinology 12(3): 405-416.
    Taylor, W. R. (1981). On preservation of color and color patterns. 3: 2-10.
    Theerakulkait, C. and Barrett, D. M. (1995). Lipoxygenase in sweet corn germ: Isolation and physicochemical properties. Journal of Food Science 60(5): 1029-1033.
    Tsukuda, N. and Amano, K. (1967). Studies on the discoloration of red fishes-IV. Discoloration of astaxanthin, tunaxanthin, and β-carotene by the tissue homogenates of fish. Bulletin of the Japanese Society of Scientific Fisheries 33: 962-969.
    Van der Salm, A. L., Martínez, M., Flik, G. and Wendelaar Bonga, S. E. (2004). Effects of husbandry conditions on the skin colour and stress response of red porgy, Pagrus pagrus. Aquaculture 241(1-4): 371-386.
    Wasson, D. H., Reppona, K. D. and Kandianis, T. M. (1991). Antioxidants to preserve rockfish color. Journal of Food Science 56(6): 1564-1566.
    Wright, W. D. (1929). A re-determination of the trichromatic coefficients of the spectral colours. Transactions of the Optical Society 30: 141-164.
    大田登原著,陳鴻興、陳君彥編譯。(2009)。基礎色彩再現工程,中議修訂二版,
    全華圖書,台北縣。
    王鑒衷。(1994)。魚類浸製標本失去銀色的原因及客服方法。生物學通報 26卷:
    第6期。
    朱祥海。(1997)。魚類學。水產出版社,台北縣。
    孫明奇,胡建中,潘思軼。(2007)。柑橘皮類胡蘿蔔素提取物穩定性研究。食品
    科學 28, No. 10
    張為憲,李敏雄,呂政義,張永和,陳昭雄,孫璐西,陳怡宏,張基郁,顏國欽,
    林志城,林慶文。(1995)。食品化學。華香園,台北市。
    張為憲,林宏基,甘子能,陳昭義,蕭寧馨。(2005)。高等食品化學。華香園,
    台北市。
    許碧真。(2011)。以非線性色彩補償技術為基礎之高動態範圍影像融合技術。國
    立中央大學碩士論文。
    劉金海,王安立,王維娜。(2005)。草金魚總色素穩定性研究(I)-日光、空氣、溫度、金屬離子對草金魚總色素穩定性的影響。水產科學 24卷: 第2期。
    劉金海,王安立,王維娜。(2005)。草金魚總色素穩定性研究(II)-酸鹼度、維生素和有機物對草金魚總色素穩定的影響。水產科學 24卷: 第3期。

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