研究生: |
林浩暉 Lin, Hao Hui |
---|---|
論文名稱: |
聲波刺激對綠豆萌芽及其幼苗生長的影響 Effects of sound stimulation on the germination and seedling growth of mung beans |
指導教授: |
徐邦達
Hsu, Ban Dar |
口試委員: | |
學位類別: |
碩士 Master |
系所名稱: |
生命科學暨醫學院 - 生物資訊與結構生物研究所 Institute of Bioinformatics and Structural Biology |
論文出版年: | 2009 |
畢業學年度: | 98 |
語文別: | 中文 |
論文頁數: | 63 |
中文關鍵詞: | 聲波刺激 、綠豆 、萌芽 、幼苗成長 |
外文關鍵詞: | Sound stimulation, Mung beans, Germination, Seedling growth |
相關次數: | 點閱:60 下載:0 |
分享至: |
查詢本校圖書館目錄 查詢臺灣博碩士論文知識加值系統 勘誤回報 |
由參考文獻得知,植物在特定頻率的聲波刺激下能較快速成長。本研究以簡單的聲波裝置,固定聲波強度在100 db下,藉由改變頻率研究聲波對於綠豆的萌芽與幼苗成長之影響。以發芽率、相對鮮重、葉面積、莖長度和主根長度當作生長指標;並且分析根、莖、葉的相對可溶性蛋白、可溶性糖、澱粉和丙二醛含量作為生化指標。實驗結果顯示,聲波對於綠豆生長確有增進作用。在生長指標上對於綠豆葉面積促進最大;也發現在聲波影響下會增加綠豆的水分蒸散速率。此外在春季時期,刺激綠豆成長可能的最佳聲波頻率為1600 Hz,但再提升頻率則會對其生長產生抑制作用;另在夏季時期,刺激綠豆生長的最佳頻率則為3200 Hz,且聲波頻率越高對於綠豆主根的生長越好。如能找出每種植物的最佳刺激頻率,未來可以運用在增加各種農業的產量。
[1]Rayleigh, L. The Theory of Sound, 2nd. ed. Macmillan:
London, (1926).
[2]Tibbitts, T. W., Langhans. R. W. Chapter 5 Controlled-
environment studies in Photosynthesis and production in
a changing environment. Chapman and Hall: New York,
(1993).
[3]Uchida, A., Yamanmoto, K. T. Effects of mechanical
vibration on seeds germination of Arabidopsis thaliana
(L.)Heynh. Plant Cell Physiol. 43(6), 647-651 (2002).
[4]Wang, B. C., Long, X., Duan, C. R., Liu, Y. Y. Response
of gerbera jamesonii acrocarpous callus stimulated by
mechanical vibration. Colloid And Surfaces B:
Biointerfaces. 21, 253-257 (2001).
[5]Wu, J., Lin, L. Elicitor-like effects of low-energy
ultrasound on plant (Panax ginseng) cells: induction of
plant defense responses and secondary metabolite
production. Biotechnol. 59, 51-57 (2002).
[6]Wu, J., Lin, L. Ultrasound-induced stress response of
Panax ginseng cells: enzymatic browning and phenolics
production. Biotechnol. 18, 862-866 (2002).
[7]Wang, B. C., Yoshikohi, A., Sakanishi, A. Carrot cell
growth response in a stimulated ultrasonic environment.
Colloid And Surfaces B: Biointerfaces. 12, 89-95 (1998).
[8]Monselise, E. B. I., Parola, A. H., Kost, D. Low-
frequency electromagnetic fields induce a stress effect
upon higher plants, as evident by the universal stress
signal, alanine. Bioelectromagnetics. 302, 427-434
(2003).
[9]David, R., Alain, V. Electromagnetic fields (900 MHz )
evoke consistent molecular responses in tomato plants.
Physiologia Plantarum. 128, 283-288 (2006).
[10]Mirta, M., Kresimir, M., Branka, P. K. Influence of
400, 900, and 1900 MHz electromagnetic fields on Lemna
minor growth and peroxidase activity.
Bioelectromagnetics. 26, 185-193 (2005).
[11]Ekici, N., Dane, F., Mamedova, L., Metin, I.,
Huseyinov, M. The effect of different musical elements
on root growth and mitosis in Onion(Allium cepa) root
apical meristem (Musical and Biological Experiment
Study). Asian Journal of Plant Science. 6, 369-373
(2007).
[12]Creath, K., Schwartz, G. E. Measuring effects of music,
noise, and healing energy using a seed germination
bioassay. Alternative And Complementary Medicine. 10,
113-122 (2004).
[13]Qin, Y. C., Lee, W. C., Choi, Y. C., Kim, T. W.
Biochemical and physiological changes in plants as a
result of different sonic exposures. Ultrasonic. 41,
407-411 (2003).
[14]Liu, Y. Y., Wang, B. C., Zhao, H. C., Duan, C. R.,
Chen, X. Alternative stress effects on Ca2+
localization in Chrysanthemum callus cells. Colloid And
Surfaces B: Biointerfaces. 22, 245-249 (2001).
[15]Wang, B. C., Zhao, H. C., Duan, C. R., Akio, S. Effects
of cell wall calcium on the growth of Chrysanthemum
callus under sound stimulation. Colloid And Surfaces B:
Biointerfaces. 25, 189-195 (2002).
[16]Wang, B. C., Shao, J., Li, B., Lian, J., Duan, C. R.
Soundwave stimulation triggers the content change of
the endogenous hormones of Chrysanthemum mature callus.
Colloid And Surfaces B: Biointerfaces. 37, 107-112
(2004).
[17]Zhao, H. C., Wang, B. C., Liu, B. A., Cai, S. X., Xi,
B. S. The effect of sound stimulation on the
permeability of K+ channel of Chrysanthemum callus
plasma. Colloid And Surfaces B: Biointerfaces. 26, 329-
333 (2002).
[18]Zhao, H. C., Wu, J., Xi, B. C., Wang, B. C. Effects of
sound-wave stimulation on the secondary structure of
plasma membrane protein of tobacco cells. Colloid And
Surfaces B: Biointerfaces. 25, 29-32 (2002).
[19]Jia, Y., Wang, B. C., Wang, X., Duan, C. R., Yoshiharu,
T., Akio, S. Influence of sound wave on the
microstructure of plasmalemma of Chrysanthemum roots.
Colloid And Surfaces B: Biointerfaces. 29, 109-113
(2003).
[20]Wang, B. C., Zhao, H. C., Liu, Y., Jia, Y., Akio, S.
The effects of alternative stress on the cell membrane
deformability of Chrysanthemum callus cells. Colloid
And Surfaces B: Biointerfaces. 20, 321-325 (2001).
[21]Jia, Y., Wang, B. C., Wang, X., Wang, D., Duan, C. R.,
Yoshiharu, T., Akio, S. Effect of sound wave on the
metabolism of Chrysanthemum roots. Colloid And Surfaces
B: Biointerfaces. 29, 115-118 (2003).
[22]Wang, B. C., Zhao, H. C., Wang, X., Duan, C. R., Wang,
D., Akio, S. Influence of sound stimulation on plasama
membane H+-ATPase activity. Colloid And Surfaces B:
Biointerfaces. 25, 183-188 (2002).
[23]Zhao, H. C., Zhu, T., Wu, J., Xi, B. S. Role of protein
kinase in the effect of sound stimulation on the PM H+-
ATPase activity of Chrysanthemum callus. Colloid And
Surfaces B: Biointerfaces. 26, 335-340 (2002).
[24]Yang, X., Wang, B. C., Duan, C. R. Effect of sound
stimulation on energy metabolism of Actinidia chinensis
callus. Colloid And Surfaces B: Biointerfaces. 30, 67-
72 (2003).
[25]Donald, F. C., JR. The induction of soluble peroxidase
activity in bean leaves by wind-induction mechanical
perturbation. American Journal of Botany. 85(11), 1586-
1591 (1998).
[26]Wang, X., Wang, B. C., Jia, Y., Liu, D., Duan, C. R.,
Yang, X., Akio, S. Effects of sound stimulation on
protective enzyme activities and peroxidase isoenzymes
of Chrysanthemum. Colloid And Surfaces B:
Biointerfaces. 63, 59-63 (2003).
[27]Li, B., Wei, J., Wei, X., Tang, K., Liang, Y., Shu, K.,
Wang, B. C. Effect on sound wave stress on antioxidant
enzyme and lipid peroxidation of Dendrobium candidum.
Colloid And Surfaces B: Biointerfaces. 63, 269-275
(2008).
[28]Wang, X., Wang, B. C., Jia, Y., Duan, C. R., Akio, S.
Effect of sound wave on the synthesis of nucleic acid
and protein in Chrysanthemum. Colloid And Surfaces
B: Biointerfaces. 29, 99-102 (2003).
[29]Shao, H., Li, B., Wang, B. C., Tang, K., Liang, Y. A
study on differentially expressed gene screening of
Chrysanthemum plants under sound stress. Comptes Rendus
Biologies. 331, 329-333 (2008).
[30]Wang, B. C., Zhang, J., Li, B., Wang, D., Duan, C. R.
Study of mRNA expression of Arabidopsis thaliana under
stimulation using modified differential display RT-PCR
with silver staining. Colloid And Surfaces B:
Biointerfaces. 40, 31-34 (2005).
[31]Jeong, M. J., Shim, C. K., Lee, J. O., Kwon, H. B.,
Kim, Y. H., Lee, S. K., Byun, M. O., Park, S. C. Plant
gene response to frequency-specific sound signals. Mol
Breeding. 21, 217-226 (2008).
[32]Bramm, J. Regulated expression of the calmodulin TCH
genes in cultured Arabidopsis cells: Induction by
calcium and heat shock. Plant Biology. 89, 3213-3216
(1992).
[33]Bramm, J., Melissa, M. L., Polisensky, D. H., Xu, W.,
Purugganan, M. M., Antosiewicz, D. M., Campbell, P.,
Johnson, K. A. Plant response to environmental stress:
regulation and functions of the Arabidopsis TCH genes.
Planta. 203, S35-S41 (1997).
[34]Melissa, M. L., Antosiewicz, D. M., Purugganan, M. M.,
Braam, J. Arabidopsis TCH3 encodes a novel Ca2+ binding
protein and shows environmentally induced and tissue-
specific regulation. The Plant Cell. 6, 1553-1565
(1994).
[35]Chehab, E. W., Eich, E., Braam, J. Thigmomorphogenesis:
a complex plant response to mechano-stimulation.
Journal of Experimental Botany. 60(1), 43-56 (2009).
[36]Telewski, F. W. A unified hypothesis of
mechanoperception in plants. American Journal of
Botany. 9(10), 1466-1476 (2006).
[37]王宏江, 王明亮, 黃維兵. 植物聲頻控制技術在棉花上的應用效
果研究. 新疆農業科學. 46(2), 415-418 (2009).
[38]王清池,周時强,李文權. 聲波與水中生物. (廈門大學海洋系
361005) 1998年第6期。
[39]Maurice J.M. Martens., Michelsen, A. Absorption of
acoustic by plant leaves. Acoustical Society of
America. 69(1) (1981).
[40]Wang, B. C., Chen, X., Wang, F., Fu, Q., Zhao, H., Ran,
L. Biological effect of sound field stimulation on
paddy rice seeds. Colloid And Surfaces B:
Biointerfaces. 32, 29-34 (2003).
[41]Zhao, H. C., Wu, J., Zheng, L., Zhu, T., Xi, B. S.,
Wang, B. C., Cai, X. S., Yonian, Y. Effect of sound
stimulation on Dendranthema marifolium callus growth.
Colloid And Surfaces B: Biointerfaces. 30, 143-147
(2003).
[42]Adams, C. A., Rinne, R. W., Fjerstad, M. C. Starch
deposition and carbohydrase activities in developing
and germinating soya bean seeds. Annals of Botany
Company. 45, 577-582 (1980).