軟骨細胞的再生以及自我修復功能差，一直是醫學發展中重要的課題，欲解決此急迫問題，我們觀察軟骨細胞在不同應力環境下的生長特性。
本研究主要規劃一維方向動態移動平台系統，我們選擇了聚二甲基矽氧烷(PDMS)當作細胞培養基材，是一種具有生物相容性且可塑性高的物質，可藉由此種特殊材料傳遞力學到細胞上，但PDMS的疏水性及其表面不具有促進細胞貼附之基團，使得此材料使用上受到限制，研究中於表面經氧電漿處理後，修飾蛋白質，運用正負電荷相吸原理能有效促進細胞貼附。系統藉由不同的應力環境給軟骨細胞的刺激，以細胞型態、外基質濃度與軟骨細胞楊氏係數來整合其生長特性。影響力學參數有很多，於研究中發現，無論任何力學參數量值大小，軟骨細胞在受到力學刺激後，與靜態環境比較之下，其軟骨細胞分泌的第一類型膠原蛋白都會有明顯提升。之後，再運用AFM量測軟骨細胞在力學刺激後的軟硬程度，實驗結果指出，經過力學刺激後細胞本身都有稍稍提高硬度，實驗推測細胞經過刺激後會變得稍微強壯，以利在受到力學刺激的環境中生長，不過這部分細節的實驗值得我們後續研究探討。

The ability of self-repaired limited in damaged chondrocytes has been an important issue in medical development.We must create more chondrocytes sources to encounter this problem. The sources of chondrocytes come into two main parts, the first one is differentiated from messenchymal stem cells, the other is culturing chondrocytes in vitro from autologous transplantation. However, if we can find a way to culture high production chondrocytes in vitro, it will provide a more directly method to solve the problem.We want the cell activity, metabolic and proliferation activities of chondrocytes staining the good status even in-vitro culture.In this paper, we design an intuitive method to enhance cells proliferation and promote extracellular matrix (ECM) synthesis by giving whole cells a homogeneous extension force at one-dimensional direction and then release it to recover original state. Due to PDMS is a hydrophobic material, we used both oxygen plasma and poly-L-lysine treatments ensuring cell adhesion result. Uniform cells were grown inside the wells and underwent different stress and cycling conditions, and it was found that tensile stress under 3% strain at 0.5Hz continuously stretching for 1 hours, and frequency for one time per three days which was most helpful for chondrocytes interior activity in plane well.Afterward we discover that cells over 5% strains dynamic condition, cell numbers and ECM synthesis would be decreased, we think this condition won’t suit for chondrocytes proliferation.

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