本論文旨在製備能穩定分散在水中且表面具有不同種類官能基之氧化鐵奈米粒子，後欲將其及應用於生物醫學領域。實驗上分別採用共沉法及熱裂解法二種合成方式。材料分析部分，使用超導量子干涉磁量儀 (Superconducting quantum interference device, SQUID)、穿透式電子顯微鏡 (Transmission electron microscope, TEM)、霍氏轉換紅外光譜儀 (Fourier transform infrared spectroscopy, FTIR)、X光粉末繞射儀 (X-ray power diffractometer, XRD)及拉曼散射 (Raman Scattering)分別進行量測氧化鐵奈米粒子的磁性、粒徑大小、表面修飾官能基及結構的鑑定。為了檢測氧化鐵奈米粒子對細胞生物相容性及磁標定量，實驗上亦將所合成之氧化鐵奈米粒子與細胞進行共培養後，分析細胞存活率以檢測奈米粒子造成的細胞毒性及細胞所胞吞磁顆粒的數量。爾後希望能將所合成之氧化鐵奈米粒子應用在生醫領域。

The magnetic nanoparticles (MNPs) have received attention during the recent few years in the biomedical field. In this thesis, Fe3O4 nanoparticles (NPs) are prepared by co-precipitation method and thermal decomposition. The Fe3O4 NPs can easily disperse in water or organic solvent. In the measurements, transmission electron microscopy (TEM) is used to characterize the size and shape of particles. The crystal structures are detected by X-ray diffraction (XRD) and Raman scattering. Magnetization is measured by superconducting quantum interference device (SQUID) magnetometer. In order to apply NPs for biomedical usages, we use 3-(4, 5-Dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay to detect the cell biocompatibility. Prussian blue staining and ferrozine-based assay are used to qualitative or quantitative analyze the magnetic labeling efficacy.

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