食用油品與我們的生活息息相關，油脂不僅是人體不可或缺的營養素之一，許多烹調方式都需要使用「油」來進行，黑心油風暴的發生不僅對我們的飲食造成衝擊，對於民眾在選購油品時也產生不少疑慮，事件的發展更凸顯出食品檢測方法的不足。
第一部份以簡單的前處理技術搭配線上(on-line)衍生氣相層析質譜儀(GC-MS)來檢測植物油中脂肪酸的組成以及反式脂肪酸的含量，油樣品與衍生化試劑四甲基氫氧化銨(TMAH，25% 甲醇溶液)同時進樣，在300℃的進樣口瞬間生成脂肪酸甲基衍生物，以此方法推算各油種脂肪酸比例，建立一套指紋圖譜系統，用以判別食用油的種類。
第二部份則以動植物固醇類來輔助食用油種的判別與摻偽，利用甲醇萃取搭配氣相層析質譜儀(GC-MS)進行分析，以質譜全掃描方式，成功定性16種化合物，在油品中發現谷固醇 (Sitosterol)、豆固醇(Stigmasterol)、菜油固醇 (Campesterol) 等多種植物固醇，作為油品標誌物，其結果進行統計分析將可增進食用油品指紋圖譜的判讀。

Edible oils play an important role for human body. They provide the essential nutrients such as fat and are widely used in the cooking and food processing.
Tainted oil scandal not only impacts our diet, but also raises the awareness for people about the quality of edible oil. This event shows that the existing food testing method is not enough. In view of this, we want to build an analytical method to figure out this problem.
There are two parts in this study. In first section, we used on-line derivatization and combined with GC/MS (gas chromatograph/mass spectrometer) to measure the fatty acid composition and the trans fatty acids content of vegetable oils. Oil samples with a derivatizing reagent (TMAH) simultaneously entering the inlet, and the fatty acid methyl esters were generated in the inlet of 300℃. In this way, we can calculate the ratio of the fatty acids and then establishing a fingerprint system to determine the type of oil
For the second part, we used sterols to assist the species discrimination and adulteration of edible oil. Utilizing methanol extraction as pretreatment method and combined with GC/MS to identify the components of edible oil. Finally, we determined 16 analytes and obtained many phytosterols, such as Sitosterol, Stigmasterol.and Campesterol, as oil marker. The results were statistically analyzed to improve the performance of oil fingerprinting.

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