本篇論文分為兩部分，第一部分是為研究在細胞中 Dynein Heavy Chain (DHC) 的生理作用。 Cytoplasmic dynein 是一種運動蛋白，靠 DHC 水解 ATP 產生能量，使 dynein 能在微管 (microtubule) 上移動運輸物質，並且和細胞分裂時胞器的位置有關。當我們在細胞中外加 100 μM vanadate (Vi) 並照射 UV 光使 DHC 裂解，觀察 DHC 被裂解後對細胞生理作用的影響。結果發現照 UV 光會使細胞大量死亡，導致無法繼續研究。
本論文之第二部分是利用大小約 80 μm 的 agarose beads 接上交聯試劑 (cross-linking reagent) 後，來標定 (pulled out) 出細胞核外膜上蛋白質。進一步用二維電泳法分析比較外膜蛋白質與原來細胞核蛋白質的成份差異。選出有顯著差異的膠點進行 in-gel digestion ，再利用 MALDI-TOF 儀器分析鑑定蛋白質的身份。鑑識出來的結果來對照這些蛋白質可能出現在細胞核膜上的位置。最明顯的就是 Lamin A這個蛋白質不會出現在標定之蛋白質裡。由此可證明這個方法不但是成功的標定出細胞核外膜上蛋白質，而且還可以了解外層核膜蛋白質的立體結構，來推測這些蛋白質在核膜上扮演了何種角色。

Asai D. J. and Koonce M. P. (2001) The dynein heavy chain: structure, mechanics and evolution. TRENDS in Cell Biology 11, 196~202

Asai D. J. and Wilkes D. E. (2004) The Dynein Heavy Chain Family. J. Eukaryot. Microbiol. 51, 23~29

Corthesy-Theulaz Irene, Pauloin Alain, and Pfeffer S. R. (1992) Cytoplasmic Dynein Participates in the Centrosomal Localization of the Golgi Complex. J. Cell Biol.118, 1333~1345

Criswell P. S. and Asai D. J. (1998) Evidence for Four Cytoplasmic Dynein Heavy Chain Isoform in Rat Testis. Mol. Biol. Cell. 9, 237~247

Dillman III J. F. , Dabney L. P. , and Pfister K. K. (1996) Cytoplasmic dynein is associated with slow axonal transport. Proc. Natl. Acad. Sci. USA. 93, 141~144

Doye Valérie and Hurt Ed (1997) From nucleoporins to nuclear pore complexes. Curr. Opin. Cell Biol. 9, 401~410

Dworetzky, S. I. , Lanford, R. E. , and Feldherr, C .M. (1988) The effects of variations in the number and sequence of targeting signals on nuclear uptake. J. Cell Biol. 107, 1279~1287.

Eiford L. A., Ow R. A. and Gibbons I. R. (1986) Specific Cleavage of Dynein Heavy Chain by Ultraviolet Irradiation in the Presence of ATP and Vanadate. J. Biol. Chem. 261, 2337~2342

Foisner Roland (2001) Inner nuclear membrane proteins and the nuclear lamina. J. Cell Sci. 114, 3791~3792

Gee M. A., Heuser J. E. and Vallee R. B. (1997) An extended microtubule-binding structure within the dynein motor domain. Nature 390, 636~639

Gibbons I. R. , Eiford A. L. , Mocz Gabor, Phillipson A. C. , Tang Wen-Jing Y., and Gibbons B. H. (1987) Photosensitized Cleavage of Dynein Heavy Chains. J. Biol. Chem. 262, 2780~2786

Graham J. M. (2001) Current Protocols in Cell Biology In: Isolation of Nuclei and Nuclear Membranes From Animal Tissues unit 3.10

Graham J., Ford T. and Rickword D. (1994) The Preparation of Subcellular Organelles from Mouse Liver in Self-Generated Gradient of Iodixanol. Anal. Biochem. 220, 367~373

Gee M. A., Heuser J. E. and. Vallee R. B (1997) An extended microtubule-binding structurewithin the dynein motor domain. Nature 390, 636~640

Holzbaur E. L. F. and Vallee R. B. (1994) Dyneins : Molecular Structure and Cellular Function. Annu. Rev. Cell Biol. 10, 339~372

King S. M. (2000) The dynein microtubule motor. Biochim. Biophys. Acta. 1496, 60~75

King S. M. (2000) AAA domains and organization of the dynein motor unit. J. Cell Sci. 113, 2521~2526

Koonce M. P. , Köhler Jana, Neujahr Ralph, Schwartz Jean-Marc, Tikhonenko Irina and Gerisch Günther (1999) Dynein motor regulation stabilizes interphase microtubule arrays and determines centrosome position. E.M.B.O. Journal. 18, 6786~6792

Korosec Thomas, Gerner Christopher and Sauermann Georg (1997) Common nuclear matrix proteins in rat tissues. Electrophoresis 18, 2109~2115

Krecic A. M. and Swanson M. S. (1999) hnRNP complexes composition, structure, and function. Curr. Opin. Cell Biol. 11, 363~371

LaMonte B. H., Wallace K. E., Holloway B. A., Shelly S. S., Ascaño Jennifer, Tokito Mariko, Winkle T. V., Howland D. S., and Holzbaur E. L. F. (2002) Disruption of Dynein/Dynactin Inhibits Axonal Transport in Motor Neurons Causing Late-Onset Progressive Degeneration. Neuron 34, 715~727

Lyman S.K., Gerace L. (2001) Nuclear pore complexes: dynamics in unexpected places. J Cell Biol. 154, 17~20

Muresan Virgil, Godek C. P., Reese T. S., and Schnapp B. J. (1996) Plus-end Motors Override Minus-end Motors during Transport of Squid Axon Vesicles on Microtubules. J Cell Biol. 135, 383~397

Nakielny Sara and Dreyfuss Gideon (1999) Transport of Proteins and RNAs in and out of the Nucleus. Cell 99, 667~690

Porter M. E.(1996) Axonemal dyneins: assembly, organization, and regulation. Curr. Opin. Cell Biol. 8, 10~17

Robinson J. T., Wojcik E. J., Sanders M. A., McGrail Maura, and Hays T. S. (1999) Cytoplasmic Dynein Is Required for the Nuclear Attachment and Migration of Centrosomes during Mitosis in Drosophila. J. Cell Biol. 146, 597~608

Sanchez Jean-Charles, Chiappe Diego, Converset Véronique, Hoogland Christine, Binz Pierre-Alain, Paesano Salvo, Appel R. D., Wang Steven, Sennitt Matthew, Nolan Anna, Cawthorne M. A., Hochstrasser D. F. (2001) The mouse SWISS-2D PAGE database: a tool for proteomics study of diabetes and obesity. Proteomics 1, 136-163

Satir Peter. (1965) Studies on cilia: II. Examination of the distal region of the ciliary shaft and the role of the filaments in motility. J. Cell Biol. 26, 805~834

Saunders W. S., Koshland Douglas, Eshel Dan, Gibbons I. R., and Hoyt M. A. (1995) Saccharomyces cerevisiae Kinesin- and Dynein-related Proteins Required for Anaphase Chromosome Segregation. J. Cell Biol. 128, 617~624

Schnapp B. J. and Reese T. S. (1989) Dynein is the motor for retrograde axonal transport of organelles. Proc. Natl. Acad. Sci. USA. 86, 1548~52

Silvanovich Andre, Li M.G. , Serr Madeline, Mische Sarah, and Hays T. S. (2003) The Third P-loop Doman in Cytoplasmic Dynein Heavy Chain Is Essential for Dynein Motor Function and ATP-sensitive Microtubule Binding. Mol. Biol. Cell. 14, 1355~1365

Soman Gopalan, Chang Y. C. and Graves D. J. (1983) Effect of Oxyanions of the Early Translation Metals on Rabbit Skeletal Muscle Phosphorylase. Biochemistry 22, 4994~5000

Summers, K. E., and Gibbons, I. R. (1971) Adenosine triphosphate-induced sliding of tubules in trypsin-treated flagella of sea-urchin sperm. Proc. Natl. Acad. Sci. USA. 68, 3092~096

Wallace D. M. (1987) Spermine precipitation of DNA. Meth. Enzymol. 152, 46

Wilson K. L., Zastrow M. S., and Lee K. K. (2001) Lamins and Disease: Insights into Nuclear Infrastructure. Cell 104, 647~650

Vallee R. B. and Sheetz M. P. (1996) Targeting of motor proteins. Science 271, 1539~1544

Vallee R. B., Williams J. C., Varma Dileep and Barnhart L. E. (2004) Dynein: An Ancient Motor Protein Involved in Multiple Modes of Transport. J Neurobiol. 58, 189~200

Xiang Xin, Beckwith S. M. and Morris N. R. (1994) Cytoplasmic dynein is involved in nuclear migration in Aspergillus nidulans. Proc. Natl. Acad. Sci. USA. 91, 2100~2104

Yamamoto Ayumu and Hiraoka Yasushi (2003) Cytoplasmic dynein in fungi: insights from nuclear Migration. J. Cell Sci. 116, 4501~4512