敗血症是指當人體的免疫不足，受感染(細菌、黴菌、病毒等病原體)所引發的複雜性、全身發炎反應，為一種不可忽視危及性命的疾病。在台灣，致死率甚至高達七成。既然細菌入侵造成感染死亡的風險高居不下，能夠及時鑑定細菌、並對症下藥成為首要其衝不可輕視任務。而目前最廣為常見的細菌檢測方法為；利用培養基進行細菌增殖，再藉由一連串生物反應；微生物的發酵、吸附特定受質、並進行還原與降解，以此鑑定生物種類，或是使用聚合酵素連鎖反應(Polymerase Chain Reaction，PCR)，質譜鑑定(Mass Spectrometry，MS)等大型儀器進行判讀、分析。雖然利用大型儀器的方法精準度高，但如若遇上培養困難、生長遲緩、無視當菌種等待時間將不可避免地成為問題。
為了改善上述耗時的問題，本次研究提出新型微流體介電泳晶片，將介電泳力(Dielecteophoresis, DEP)加進DLD裝置中(deterministic lateral displacement)。傳統DLD裝置藉由在晶片中設立許多柱子結構，當包含不同大小粒子的溶液由入口注入後，藉由慣性撞擊，使之慢慢跨越流線，達到分離的效果。然而隨著尺寸效應(Scaling Law)的引響，在微流體系統中慣性力將不再佔有主導地位，故本次研究嘗試將柱子以電極代替，用介電泳代替慣性力提升分選效率。最後在晶片末端利用SERS 指紋識別技術檢測微流道中的細菌。

Sepsis refers to the complex and systemic inflammatory response caused by infection (bacteria, mold, virus and other pathogens) when the body's immune system is insufficient. It is a life-threatening disease that cannot be ignored. In Taiwan, the fatality rate is as high as 70%. Since the risk of infection and death caused by bacterial invasion remains high, being able to identify bacteria in a timely manner and prescribe the right medicine has become the primary task that cannot be underestimated. At present, the most common bacterial detection methods are: using culture media to multiply bacteria, and then through a series of biological reactions; fermentation of microorganisms, adsorption of specific substrates, reduction and degradation to identify biological species, or use polymerization Enzyme chain reaction (Polymerase Chain Reaction, PCR), mass spectrometry (MS) and other large instruments for interpretation and analysis. Although the method using large instruments is highly accurate, it will inevitably become a problem if it encounters cultivation difficulties, growth retardation, and ignoring the waiting time of the strain.
In order to improve the above-mentioned time-consuming problem, this research proposes a new type of microfluidic dielectrophoresis wafer, which adds dielectrophoresis (Dielecteophoresis, DEP) into the DLD device (deterministic lateral displacement). The traditional DLD device uses many pillar structures in the chip. After the solution containing particles of different sizes is injected from the inlet, the inertial impact causes it to slowly cross the streamline to achieve the effect of separation. However, with the influence of the Scaling Law, the inertial force will no longer dominate in the microfluidic system. Therefore, this study tried to replace the column with an electrode and use dielectrophoresis to replace the inertial force to improve the sorting efficiency. Finally, SERS fingerprint recognition technology is used to detect bacteria in the micro flow channel at the end of the wafer.

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