We use laser ablation inductively coupled mass spectrometry (LA-ICP-MS) to establish mapping technique for the analysis of elements in environmental and biological samples, which provides high sample throughput, high sensitivity, accuracy and precision of the analytical data for trace and ultra-trace multi-element analysis of solid samples. In this study, a laser beam at a fixed speed (line scan mode) moving along the sample has been used in the LA-ICP-MS mapping technique. MATLAB is utilized to process data, to produce two- or three-dimensional image and to integrate counts for quantitative or qualitative analysis of samples. In addition, a comparison of the proposed method with the laser beam in a fixed position on the sample (grid mode) in analyzing standard samples and real samples are also performed. A better linearity of calibration curves established from standard samples is shown in line scan mode, but similar elemental concentrations are observed by both methods. The proposed mapping technique by LA-ICP-MS is successfully applied in aerosol particulate samples and a slice of lung tissues.

Ammann A. A.; Inductively coupled plasma mass spectrometry (ICP MS): a versatile tool, Journal of Mass Spectrometry, 2007, 42, 419–427.

Allen A.G.; E. Nemitz; J.P. Shi; R.M. Harrison; J.C. Greenwood; Size distributions of trace metals in atmospheric aerosols in the United Kingdom, Atmospheric Environment, 2001, 35, 4581–4591.

Becker J. S.; Applications of inductively coupled plasma mass spectrometry and laser ablation inductively coupled plasma mass spectrometry in materials science, Spectrochimica Acta Part B, 2002, 57, 1805–1820.

Becker J. S.; M. V. Zoriy; M. Dehnhardt; C. Pickhardt; K. Zilles; Copper, zinc, phosphorus and sulfur distribution in thin section of rat brain tissues measured by laser ablation inductively coupled plasma mass spectrometry: possibility for small-size tumor analysis, Journal of Analytical Atomic Spectrometry, 2005, 20, 912–917.

Becker J. S.; M. Zoriy; B. Wu; A. Matusch; J. S. Becker; Imaging of essential and toxic elements in biological tissues by LA-ICP-MS, Journal of Analytical Atomic Spectrometry, 2008, 23, 1275–1280.

Bellotto V. R.; N. Miekeley; Improvements in calibration procedures for the quantitative determination of trace elements in carbonate material (mussel shells) by laser ablation ICP-MS, Fresenius Journal of Analytical Chemistry, 2000, 367, 635–640.

Baker S. A.; M. Bi; R. Q. Aucelio; B. W. Smith; J. D. Winefordner; Analysis of soil and sediment samples by laser ablation inductively coupled plasma mass spectrometry, Journal of Analytical Atomic Spectrometry, 1998, 14, 19–26.

Carter J.D.; A. J. Ghio; J. M. Samet; R. B. Devlin; Cytokine Production by Human Airway Epithelial Cells after Exposure to an Air Pollution Particle Is Metal-Dependent, Toxicology and Applied Pharmacology, 1997, 146, 180–188.

Carvalho M. L.; T. Magalhães; M. Becker; A. von Bohlen; Trace elements in human cancerous and healthy tissues: A comparative study by EDXRF, TXRF, synchrotron radiation and PIXE, Spectrochimica Acta Part B, 2007 62, 1004–1011.

Craig C. A.; K. E. Jarvis; L. J. Clarke; An assessment of calibration strategies for the quantitative and semiquantitative analysis of calcium carbonate matrices by laser ablationinductively coupled plasma-mass spectrometry (LA-ICP-MS), Journal of Analytical Atomic Spectrometry, 2000, 15, 1001-1008.

Cui X.; R. Okayasu; Arsenic accumulation, elimination, and interaction with copper, zinc and manganese in liver and kidney of rats, Food and Chemical Toxicology, 2008, 46, 3646–3650.

DE PONTUAL H.; F. LAGARDÈRE; H. TROADEC; A. BATEL; Y. DÈSAUNAY; C. KOUTSIKOPOULOS; Otoliths imprinting of sole (Solea solea) from the Bay of Biscay: a tool to discriminate individuals from nursery origins, Oceanologica Acta, 1999, 23, 497-513.

Durrant S. F.; N. I. Ward; Recent biological and environmental applications of laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS), Journal of Analytical Atomic Spectrometry, 2005, 20, 821–829.

Durrant S. F.; Laser ablation inductively coupled plasma mass spectrometry: achievements, problems, prospects, Journal of Analytical Atomic Spectrometry, 1999, 14, 1385–1403.

Ekinci R.; N. Ekinci; An alternative method for the determination of element concentrations in schizophrenic, lung cancer and leukaemia patient bloods, Journal of Quantitative Spectroscopy & Radiative Transfer, 2003, 86, 379– 385.

Fallon S. J.; M. T. McCulloch; R. van Woesik; D. J. Sinclair; Corals at their latitudinal limits: laser ablation trace element systematics in Porites from Shirigai Bay, Japan, Earth and Planetary Science Letters, 1999, 172, 221–238.

Farrell A. P.; A. H. Hodaly; S. Wang; Metal Analysis of Scales Taken from Arctic Grayling, Archives Environmental Contamination Toxicology, 2000, 39, 515–522.

Feldmann J.; A. Kindness; P. Ek; Laser ablation of soft tissue using a cryogenically cooled ablation cell, Journal of Analytical Atomic Spectrometry, 2002, 17, 813–818.

Fernández B.; F. Claverie; C. Pécheyran; O. F. X. Donard; Solid-spiking isotope dilution laser ablation ICP-MS for the direct and simultaneous determination of trace elements in soils and sediments, Journal of Analytical Atomic Spectrometry, 2008, 23, 367-377.

Ghazi A. M.; S. Shuttleworth; S. J. Angulo; D. H. Pashley; New applications for laser ablation high resolution ICPMS (LA-HRICP-MS): Quantitative measurements of gallium diffusion across human root dentin, Journal of Analytical Atomic Spectrometry, 2000, 15, 1335-1341.

Ghio A. J.; J. Stonehuerner; L. A. Dailey; J. D. Carter; Metals associated with both the water-soluble and insoluble fractions of an ambient air pollution particle catalyze an oxidative stress, Inhalation Toxicology, 1999, 11, 37–49.

Gligorovski S.; J. T. V. Elteren; I. Grgić; A multi-element mapping approach for size-segregated atmospheric particles using laser ablation ICP-MS combined with image analysis, Science of The Total Environment, 2008, 407, 594 – 602.

González J. J.; A. Fernández; X. Mao; R. E. Russo; Scanning vs. single spot laser ablation (λ=213 nm) inductively coupled plasma mass spectrometry, Spectrochimica Acta Part B, 2004, 59, 369–374.

Gray A. L.; Solid sample introduction by laser ablation for inductively coupled plasma source mass spectrometry, Analyst, 1985, 110, 551-556.

Guillong M.; D. Günther; Effect of particle size distribution on ICP-induced elemental fractionation in laser ablation-inductively coupled plasma-mass spectrometry, Journal of Analytical Atomic Spectrometry, 2002, 17, 831–837.

Günther D.; B. Hattendorf; Solid sample analysis using laser ablation inductively coupled plasma mass spectrometry, Trends in Analytical Chemistry, 2005, 24, 255-265.

Jiménez M. S.; M. T. Gómez; J. R. Castillo; Multi-element analysis of compost by laser ablation-inductively coupled plasma mass spectrometry, Talanta, 2007, 72, 1141–1148.

Jackson B.; S. Harper; L. Smith; J. Flinn; Elemental mapping and quantitative analysis of Cu, Zn, and Fe in rat brain sections by laser ablation ICP-MS, Analytical and Bioanalytical Chemistry, 2006, 384, 951–957.
Kanicky V.; J. M. Mermet; Use of a single calibration graph for the determination of major elements in geological materials by laser ablation inductively coupled plasma atomic emission spectrometry with added internal standards, Fresenius Journal of Analytical Chemistry, 1999, 363, 294–299.

Kindness A.; C. N. Sekaran; J. Feldmann; Two-Dimensional mapping of copper and zinc in liver sections by laser ablation–inductively coupled plasma mass spectrometry; Clinical Chemistry 49, 2003, 11, 1916–1923.

Kubala-Kukuś A.; J. Braziewicz; D. Banaś; U. Majewska; S. Góźdź; A. Urbaniak; Trace element load in cancer and normal lung tissue, Nuclear Instruments and Methods in Physics Research B, 1999, 150, 193-199.

Latkoczy C.; Y. Müller; P. Schmutz; D. Günther; Quantitative element mapping of Mg alloys by laser ablation ICP-MS and EPMA, Applied Surface Science, 2005, 252, 127–132.

Lobinski R.; C. Moulin; R. Ortega; Imaging and speciation of trace elements in biological environment, Biochimie, 2006, 88, 1591–1604.

Majewska U.; D. Banaś; J. Braziewicz; S. Góźdź; A. Kubala-Kukuś; M. Kucharzewski; Trace element concentration distributions in breast, lung and colon tissues, Physics Medicine and Biology, 2007, 52, 3895–3911.

Milton D. A.; S. R. Chenery; The effect of otolith storage methods on the concentrations of elements detected by laser-ablation ICPMS, Journal of Fish Biology, 1998, 53, 785–794.

Morrison G.; O. S. Fatoki; S. Linder; C. Lundehn; Determination of heavy metal concentrations and metal fingerprints of sewage sludge from eastern cape province, south Aferica by inductively coupled plasma –mass spectrometry (ICP-MS) and laser ablation-inductively coupled plasma –mass spectrometry (LA-ICP-MS), Water, Air, and Soil Pollution, 2004, 152, 111–127.

OkudaT.; J. Kato; J. Mori, M. Tenmoku; Y. Suda; S. Tanaka; K. He; Y. Ma; F. Yang; X. Yu; F. Duan; Y. Lei; Daily concentrations of trace metals in aerosols in Beijing, China, determined by using inductively coupled plasma mass spectrometry equipped with laser ablation analysis, and source identification of aerosols, Science of the Total Environment , 2004, 330, 145–158.

Prohaska T.; C. Latkoczy; G. Schultheis; M. Teschler-Nicola; G. Stingeder; Investigation of Sr isotope ratios in prehistoric human bones and teeth using laser ablation ICP-MS and ICP-MS after Rb/Sr separation, Journal of Analytical Atomic Spectrometry, 2002, 17, 887–891.

Rauch S.; G. M. Morrison; M. Moldovan; Scanning laser ablation-ICP-MS tracking of platinum group elements in urban particles, The Science of the Total Environment , 2002,286, 243-251.

Richardson C. A.; S. R. N. Chenery; J. M. Cook; Assessing the history of trace metal (Cu, Zn, Pb) contamination in the North Sea through laser ablation—ICP-MS of horse mussel Modiolus modiolus shells, Marine Ecology Progress Series, 2001, 211, 157-167.

Rodushkina I.; M. D. Axelsson; Application of double focusing sector field ICP-MS for multielemental characterization of human hair and nails. Part I. Analytical methodology, The Science of the Total Environment , 2000, 250, 83-100.

Russo R. E.; X. Mao; H. Liu; J. Gonzalez; S. S. Mao; Laser ablation in analytical chemistry—a review, Talanta, 2002, 57, 425–451.

Singh M.; P. A. Jaques; C. Sioutas; Size distribution and diurnal characteristics of particle-bound metals in source and receptor sites of the Los Angeles Basin, Atmospheric Environment, 2002, 36, 1675–1689.

Spears D.A.; The use of laser ablation inductively coupled plasma-mass spectrometry (LA ICP-MS) for the analysis of fly ash, Fuel, 2004, 83, 1765–1770.

Suzuki Y.; I. Watanabe; T. Oshida; Y. J. Chen; L. K. Lin; Y. H. Wang; K. C. Yang; K. Kuno; Accumulation of trace elements used in semiconductor industry in Formosan squirrel, as a bio-indicator of their exposure, living in Taiwan, Chemosphere, 2007, 68, 1270–1279.

Tan C.; H. Chen; C. Xia; Early prediction of lung cancer based on the combination of trace element analysis in urine and an Adaboost algorithm, Journal of Pharmaceutical and Biomedical Analysis, 2009, 49, 746–752.

Tanaka S.; N. Yasushi; N. Sato; T. Fukasawa; S. J. Santosa; K. Yamanaka; T. Ootoshi; Rapid and simultaneous multi-element analysis of atmospheric particulate matter using inductively coupled plasma mass spectrometry with laser ablation sample introduction, Journal of Analytical Atomic Spectrometry, 1998, 13, 135–140.

Thompson M.; S. Chenery; L. Brett; Calibration Studies in Laser Ablation Microprobe – Inductively Coupled Plasma Atomic Emission Spectrometry, Journal of Analytical Atomic Spectrometry, 1989, 4, 11-16.

Toland H.; B. Perkins; N. Pearce; F. Keenan; M. J. Leng; A study of sclerochronology by laser ablation ICP-MS, Journal of Analytical Atomic Spectrometry, 2000, 15, 1143-1148.

Triolo L.; A. Binazzi; P. Cagnetti; P. Carconi; A. Correnti; E. De Luca; R. Di Bonito; G. Grandoni; M. Mastrantonio; S. Rosa; M. Schimberni; R. Uccelli; G. Zappa; Air pollution impact assessment on agroecosystem and human health characterisation in the area surrounding the industrial settlement of Milazzo (Italy): a multidisciplinary approach, Environ Monit Assess, 2007, 140, 191–209.

Vanoeteren C.; R. Cornelis; J. Versieck; Evaluation of trace elements in human lung tissue I. Concentration and distribution, Science of The Total Environment, 1986, 54, 217-230.

Wagner B., S. Garboś; E. Bulska; A. Hulanicki; Determination of iron and copper in old manuscripts by slurry sampling graphite furnace atomic absorption spectrometry and laser ablation inductively coupled plasma mass spectrometry, Spectrochimica Acta Part B, 1999, 54, 797-804.

Wanner B.; Ch. Moor; P. Richner; Br¨onnimann; B. Magyar; Laser ablation inductively coupled plasma mass spectrometry_LA-ICP-MS.for spatially resolved trace element determination of solids using an autofocus system, Spectrochimica Acta Part B, 1999, 54, 289-298.

Zoriy M. V.; J. S. Becker; Imaging of elements in thin cross sections of human brain samples by LA-ICP-MS: A study on reproducibility, International Journal of Mass Spectrometry, 2007, 264, 175–180.

Zoriy M.V.; M. Dehnhardt; G. Reifenberger; K. Zilles ; J.S. Becker; Imaging of Cu, Zn, Pb and U in human brain tumor resections by laser ablation inductively coupled plasma mass spectrometry, International Journal of Mass Spectrometry, 2006, 257 27–33.

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