桿狀病毒/昆蟲細胞系統已經被廣泛的應用在重組蛋白質的生產上，但傳統的桿狀病毒感染後會造成細胞溶裂，而使得表現出的重組蛋白質流失到培養基中。為了改善這個缺點，本論文由兩個方向研究非溶裂桿狀病毒表現系統。首先，中研院分生所趙裕展博士建構出一種重組桿狀病毒。在貼附培養的情況下，此病毒感染宿主昆蟲細胞不會造成昆蟲細胞溶裂。而在本論文中，我們嘗試將此一非溶裂昆蟲細胞/桿狀病毒表現系統轉移到旋轉瓶的懸浮培養。
另外一方面，有文獻利用桿狀病毒作為載具，將外來基因送入哺乳動物細胞表現，且不會對哺乳動物細胞的生長造成影響。因此，本論文中討論一些影響蛋白質表現量與延長表現的參數如:培養基的更換、重複感染、病毒感染方式。藉此試著建立一非溶裂哺乳動物細胞/桿狀病毒表現系統。

Baculovirus/insect cell system has been widely used for recombinant protein production, but traditional system eventually resulted in cell lysis, so that the expressed recombinant protein was lost into medium. To improve this, the thesis researched the non-lytic baculovirus expression system by two different ways. First, Dr. Yu-Chan Chao at the Institute of Molecular Biology, Academia Sinica, constructed a recombinant baculovirus. In the adherent culture, the virus did not result in cell lysis after the insect cells were infected. In the thesis, we tried to transfer the novel non-lytic system to suspension culture in spinner flask.
Second, some reports utilized the baculovirus as a gene delivery vehicle into mammalian cells and did not have any bad effect on the growth of mammalian cell. So, the thesis tried to develop the non-lytic mammalian/baculovirus expression system by investigated the parameters influencing the level and the duration of the expression, such as medium exchange, superinfection and virus transduction strategies.

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