本論文主要研究利用簡單且體積小的光學裝置，並以光學機制作為量測人類血清白蛋白(human serum albumin, HSA)之生物感測器。
在本研究中之生物感測器製備方式，是將玻璃洗淨後，經 3-aminopropyltriethoxysilane (APTES) 或1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC)/N-Hydroxysuccinimide (NHS)改質玻璃表面，使玻璃表面具有生物相容性後，而後依序接合上人類血清白蛋白抗體 (anti-HSA, AHSA)、小牛血清蛋白阻斷(bovine serum albumin, BSA)，及不同濃度之HSA，最後修飾上具 655 nm發光波長的硒化鎘(CdSe) / 硫化鋅(ZnS)量子點(QD)，吹乾後，再以405 nm雷射激發量子點，使之發出655 nm螢光並以感光二極體(photodiode)量取接收光量後產生之電流，藉此作為人HSA濃度之定量檢測。
在以感光二極體擷取 QDs 所發出螢光，進行HSA生物感測器定量分析上，乃利用半導體參數分析儀(semiconductor parameter analyzer)，在直流電下測量由不同濃度之HSA與其接合之QDs所貢獻之電流變化量，來定量分析HSA之濃度。結果顯示當HSA的濃度越高，所產生的光電流也就越大，並且在2x10-4 mg/ml到2x10-1 mg/ml之間呈線性關係，其HSA偵測極限為3.2x10-5 mg/ml。
本研究利用光學機制，藉由量測接合HSA及QDs後所發出之螢光強度的改變，定量HSA濃度，並成功驗證此機制之可行性，跟傳統螢光式的光學生物感測器相比，本研究獨特之處在於此感測器的光源為二極體雷射，而光偵測器為感光二極體，兩者皆為體積小、低成本的電子元件，並可搭配透鏡與濾鏡，組成簡單且攜帶方便的光學系統，此簡易的光學系統具有易整合於生物晶片、分析結果可即時輸出、低成本等優點，對蛋白質檢測上有極大應用潛力。

In this study, an optical biosensor for human serum albumin (HSA) detection was demonstrated utilizing a simple and small volume optical system.
For the fabrication process of the optical biosensor, the glass sensing surface was modified by 3-aminopropyltriethoxysilane (APTES) or 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) / N-Hydroxysuccinimide (NHS) to improve the biocompatibility. Following that, the sensing surface was modified with anti-human serum albumin (AHSA), blocked by bovine serum albumin (BSA), conjugated with targeted HSA, and finally bonded with AHSA-modified quantum dots (emission wavelength : 655 nm) for HSA detection after N2 drying.
The photocurrent contributed by various concentrations of HSA was measured and quantified by semiconductor parameter analyzer under DC condition in this work. Results showed that the photocurrent increased linearly as the HSA conjugation increased from 2x10-4 mg/ml to 2x10-1 mg/ml, with a detection limit of 3.2□10-5 mg/ml.
In summary, this study has successfully demonstrated the feasibility of utilizing optical mechanism (by measuring the photocurrent change of photodetector induced by fluorescence intensity change before and after HSA, and QDs conjugating) to quantify human serum albumin (HSA) concentration. Compared to the traditional fluorescence biosensors, both diode laser light source and photodetector were low-cost and small-volume electronics in this work. This portable, simple optical system consisted of lens, filter, diode laser, and photodetector, exhibiting advantages of ease to be integrated with biochips, timely output, and low cost, that can be used for future protein detection.

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