對於大部分的多細胞動物，保持中樞神經系統正常運作絕對是攸關生死的課題之一，萬一中樞神經系統受到了損傷，動物們最好有方法讓受損的部位再生回來。再生是一個極有趣的生物現象，但目前我們對它細胞層次以及分子層次的機制不太了解。相對於人類的中樞神經系統無法再生，斑馬魚在中樞神經系統上的再生能力是非常強的，它可以再生多種中樞神經系統的器官及組織，包括小腦在內，所以拿它來做為研究中樞神經系統修復的機制是再適合不過了。在本研究中，我們使用了三種方式來分析斑馬魚小腦創傷修復過程的微陣列晶片數據，分別是時間序列分析、訊息路徑富集分析、轉錄因子富集分析、動態蛋白質互動網路分析。透過這些分析，我們希望回答的問題是那些訊息路徑調控了小腦部位受損組織的復原進程。從分析結果中，我們的確看到了許多顯著的訊息路徑在創傷復原的過程裡以不同的動態模式活躍著。此外，我們也發現一些轉錄因子可能維繫著不同動態模式的訊息路徑間的關聯性。這些發現讓我們更為清楚腦部創傷復原的分子機制，使我們朝人類中樞神經系統再生療法的目標更邁進了一步。

The central nervous system (CNS) is the most important system to most of multicellular animals. Once the CNS gets damaged, animals had better regenerate it back. Regeneration is one of the most interesting biological phenomena but its molecular and cellular mechanisms are still unclear. Danio rerio is a good model to investigate these mechanisms since it can regenerate many types of CNS tissues, including cerebellum, while we humans cannot. In this study, we applied several frequently-adopted methods such as time series analysis, pathway enrichment analysis, and promoter analysis to the temporal microarray profile of zebrafish after traumatic brain injury, as well as constructed a wound healing-related protein-protein interaction (PPI) network of zebrafish with the dynamic cellular model and system identification approaches. According to these analyses, many significant pathways and crucial transcription factors in wound healing process were identified. From these important results, we can gain more insights into the specific molecular mechanisms in CNS regeneration process and make human CNS regeneration more feasible in the future.

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