本研究創建一個簡單的整合乳化液滴生成與液滴篩選之微流道平台，應用於體外生殖技術。微流體晶片主要分成四個部分：(1)微液滴生成且分離生殖細胞(2)篩選區(3)儲存區(4)廢液區。
研究上分三個步驟。第一階段設計乳化液滴生成結構並提高檢體分離率，透過微量注射式幫浦(syringe pump)調控兩種不相溶的流體間的體積流率比，在油浴流道中生成均一粒徑乳化液滴 (Water-in-oil droplets, W/O)。本研究成功利用三入一出結構晶片，藉由流體聚焦生成可控制粒徑的乳化液滴，同時使用聚苯乙烯球(PS bead)模擬卵母細胞在流道入口的動態情況，並改以在出口端使用拉力驅動模式(Push-pull)大幅增加PS beads進入流道內的數量且大幅減少生殖細胞的損耗。
第二階段設計液滴儲存區，使用界面活性劑增加液滴穩定效果與進行細胞毒性測試。第三階段整合氣動閥控制微流道的開關，成功分離細胞於成長所需培養液內，形成乳化液滴，將其儲存於螺旋結構儲存區。以利生殖細胞操作與培養，具有製作簡單、操控便利、降低細胞培養成本、防止汙染風險、生物相容性等優點。並於活體顯微鏡上操作與觀測，以達到Lab-on-a-chip的目標。

The study created a simple microfluidic platform that uses with in-vitro fertilization (IVF). It has four parts including: (1) Microdroplets generation and cell separation, (2) Sorting area, (3) Storage area, and (4) Waste area. In this study, it is divided into three steps as follow.
First, the polydimethylsiloxane (PDMS) flow-focusing microfluidic device was used for micrometer-size medium-in-oil microdroplets generation. The volume of microdroplets can be controlled by adjusting the flow rates of both oil and medium solutions. The microdroplets formation by "push-pull" system significantly reduces the loss of embryos, and micro-pneumatic valve to control the flow channel switching and also successfully isolated microdroplets with embryo cells to the storage area.
The second step, storage area be design, then mono-dispersed microdroplets keep the stability by detergent and also to test for the detergent toxicity. The third step, the air valve was control sorting cell encapsulation into the storage area. It reduces medium volumes, cell culture cost, evaporation problem and prevents contamination risk. This droplet-based microfluidics system provides a dynamic and non-continuous culture condition for mimicking the in vivo cell development IVF research.

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