植物在季節轉換、老化、果實成熟和遭受環境逆境都會照成細胞內的葉綠素分解，導致綠色消失。。植物葉綠素分解途徑有pheophorbide a氧化酶路徑和過氧化酶路徑二種。其中以Pheophorbide a氧化酶路徑路徑較常見於大部分植物包括藻類上。植物在進行程序化細胞死亡時常伴隨葉綠素的分解，兩種葉綠素分解在程序化細胞死亡都有被使用。Pheophorbide a氧化酶路徑主要在老化造成的程序化細胞死亡 ; 過氧化酶路徑主要出現在細胞壞死、病原感染和環境逆境引發的高敏感反應所造成的程序化細胞死亡造成之葉綠素褪色。不同類型植物的程序化細胞死亡的有不同的形態特色。本研究是探討熱處理的小球藻照光培養後，會導致程序化細胞死亡。我們以高效能液層析儀發現葉綠素的分解是經由過氧化酶路徑。穿透式電子顯微鏡和核酸螢光染色﹙DAPI和Acrindine orange) 型態上非常類似於植物高敏感反應引發的程序化細胞死亡。葉綠體在整個熱處理後光照引發的程序化細胞死亡是扮演協助粒腺體的角色。

The loss of chlorophyll from plants is induced by transition of the season , senescence , fruit maturation or sufferance from environmental stress . The chlorophyll degradation is two pathway including pheophorbide a oxygenase pathway and peroxidase pathway . Pheophorbide a oxygenase pathway is popular in most plants and algae . The programmed cell death and the degradation of chlorophyll is intermix ; The degradation of chlorophyll is according to pheophorbide a oxygenase pathway and peroxidase pathway . The pheophorbide a oxygenase pathway is mainly discovered in the programmed cell death of senescence ; the peroxidase pathway is mainly discovered in necrosis and the programmed cell death of hypersensitive response induced from phage and environmental stress . The different programmed cell death has different morphological characters . In this study , we want to study the programmed cell death of heat-treated chlorella after incubated in light condition . We discovery the degradation of chlorophyll is according to the peroxidase pathway by HPLC . We discovery the morphological characters by TEM and nuclear florescence staining (DAPI and Acrindine orange) is similar as morphological characters of the programmed cell death induced hypersensitive response . The chloroplast cooperate with mitochondria in the programmed cell death of heat – treaed chlorella .

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