新冠肺炎的的大流行與快速傳播在世界各地造成大量生命的損失。為了有效解決，多種疫苗快速地被研發並施打，而隨著疫苗的涵蓋率逐漸普及，仍持續出現突破性感染，因此疫苗接種者是否真的具備足夠保護力就成了更重要的課題。本研究中首先專注於抗體生成相關的分析。在第一部分裡，我們收集施打兩劑AZ疫苗的血液樣本，透過酵素結合免疫吸附分析法 (ELISA) 來檢測抗體含量，另外使用側向流體免疫分析法 (LFIA) 來提供更快速簡單檢測抗體含量的方法。在另一方面，我們也檢測了孕婦施打疫苗後的中和抗體是否會經由胎盤轉移給胎兒，還有其中和抗體是否能用以對抗Delta變異株，以及在孕期同時施打其它疫苗是否會影響中和抗體的生成效率，以此做為孕婦施打COVID-19疫苗相關方針的參考依據。而除了抗體檢測以外，本研究也希望能找到特定生物標記，用以代表或預測疫苗接種者的免疫反應。在這裡以血液中IFN-γ做為檢測標的，將其與抗體生成進行相關性分析。在後半部分的研究中，更加入了細胞免疫的分析，使用QuantiFERON SARS-CoV-2套組，檢測以抗原刺激T細胞後所釋放之IFN-γ含量，作為判斷細胞免疫反應高低的依據。綜合上述，本研究的目的便是透過對疫苗接種者後天免疫反應完整的分析來評估疫苗效力，促進疫苗的改良及優化，最終達到有效控制疫情的效果。

COVID-19 has caused significant loss of life around the world. To solve this problem, a variety of vaccines have been rapidly developed and administered. As the coverage of vaccines gradually popularized, breakthrough infections continue to occur. Therefore, whether the vaccine recipients obtain sufficient protection has become an even more important issue. In this study, we first focus on antibody production related analysis. In the first part, blood samples were collected after two doses of AZ vaccines. The antibody level was mainly detected by enzyme-linked immunosorbent assay (ELISA), and the lateral flow immunoassay (LFIA) was used to provide a more rapid and simple method. On the other hand, we tested whether the neutralizing antibody of pregnant women would be transferred to their fetus. Also, we would like to know if the neutralizing antibodies they produced can fight against SARS-CoV-2 Delta variant, and whether having other vaccines during pregnancy would influence the effectiveness of neutralizing antibody production. These results can be utilized as the reference of pregnant woman having COVID-19 vaccines. In addition to antibody detection, we also tried to find specific biomarkers that can represent or predict the immune response of vaccine recipients. Here, IFN-γ in blood is used as the detection target, and the correlation between it and antibody production is analyzed. In the subsequent experiments, cellular immunity was analyzed through QuantiFERON SARS-CoV-2 by measuring the antigen-induced-IFN-γ released. Based on the above, the purpose of this study is to evaluate vaccine efficacy through a complete analysis of the adaptive immune response of vaccine recipients, further promote vaccine improvement and optimization, and ultimately achieve the effect of effectively controlling the pandemic.

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