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研究生： 鍾明翰
論文名稱： 以三聚磷酸離子交聯聚乙二醇接枝幾丁聚醣共聚物做為傷口修復材料之研究
The study of PEG-Chitosan ionic cross-linked with triphosphate as wound healing material
指導教授： 朱一民
口試委員: 鍾次文
魏毓宏
學位類別： 碩士
Master
系所名稱： 工學院 - 化學工程學系
Department of Chemical Engineering
論文出版年： 2011
畢業學年度： 99
語文別： 中文
論文頁數： 76
中文關鍵詞： 幾丁聚醣 、三聚磷酸 、傷口敷料
相關次數： 點閱：2  下載：0 
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    • 幾丁聚醣(Chitosan)為具有良好生物相容性、生物可降解性的天然高分子，其止血、抗菌的特質適合做為傷口敷料。而聚乙二醇(Poly(ethylene glycol), PEG )為FDA認可的生醫材料，是一具有高度親水性的合成高分子。聚磷酸除了具有抗菌及持水等性質，還可做為離子交聯劑來穩固生醫高分子的結構。
    在本研究中，利用mPEG加以改質修飾chitosan，以增加chitosan的親水性，並以冷凍乾燥法製備出海綿狀薄膜，再與三聚磷酸(triphosphate, TPP) 離子交聯形成一水膠體薄膜做為濕潤型敷料。
    經過mPEG改質後的chitosan (mPEG-Chit)，其結晶結構被破壞而導致熱穩定性變差。但經TPP離子交聯後所製備出的mPEG-Chit薄膜，其親水性、含水率、膨潤率皆有所提升，並且在15天內都能保持穩定的中性狀態。從SEM的結果顯示，mPEG-Chit薄膜在經TPP交聯後其表面結構變得較不規則且孔洞有縮小的現象。另外mPEG-Chit其生物相容性較chitosan更為良好，並在老鼠傷口修復實驗中，未發現發炎或過敏反應，且癒合後的新生膠原蛋白纖維組織排列更為整齊，其中又以經17.8%TPP交聯之mPEG-Chit 2水膠體敷料有最佳的復原效果。綜合以上實驗結果得知，mPEG-Chit經TPP交聯的水膠體敷料擁有應用在幫助傷口修復的潛力。

    摘要 I Abstract II 圖目錄 VI 表目錄 VIII 第一章 文獻回顧 1 1.1 組織工程與生醫材料 1 1.1.1 組織工程的定義 1 1.1.2 生醫材料 2 1.1. 3 生物可降解性高分子 3 1.2 皮膚 6 1.2.1 皮膚的構造與功能 6 1.2.2 傷口癒合機制 8 1.3 敷料 10 1.3.1 敷料的定義 10 1.3.2 理想敷料的條件 10 1.3.3 敷料的種類 10 1.4 幾丁聚醣 13 1.4.1幾丁聚醣的來源與製備 13 1.4.2幾丁聚醣的特性與應用 15 1.4.3 幾丁聚醣於生醫材料的用途 16 1.4.4 幾丁聚醣的改質 18 1.5 聚磷酸 21 1.5.1 聚磷酸的特性與應用 21 1.5.2 聚磷酸鹽類於生醫材料的用途 22 第二章 研究目的與動機 22 第三章 實驗藥品與儀器 22 3.1 實驗藥品 22 3.2 實驗儀器 22 第四章 實驗方法與步驟 22 4.1 實驗架構 22 4.2 製備mPEG-COOH 22 4.3 製備mPEG-Chitosan 22 4.4 製備mPEG-Chitosan水膠體薄膜 22 4.5 材料性質鑑定方法 22 4.5.1核磁共振光譜分析(NMR) 22 4.5.2紅外線光譜分析(FT-IR) 22 4.5.3熱性質分析 22 4.5.4表面微結構分析 22 4.5.5含水率與膨潤率測定 22 4.5.6 pH值測定 22 4.5.7體外重量損失測試 22 4.6生物相容性分析 22 4.6.1細胞毒性試驗(MTT assay) 22 4.6.2細胞貼附實驗 22 4.6.3材料與細胞生長情形觀察 22 4.7動物實驗 22 4.8組織切片染色觀察 22 第五章 結果與討論 22 5.1 mPEG-COOH結構鑑定 22 5.1.1核磁共振光譜分析 22 5.1.2紅外線光譜分析 22 5.2 mPEG-Chitosan結構與性質鑑定 22 5.2.1核磁共振光譜分析 22 5.2.2紅外線光譜分析 22 5.2.3 mPEG接枝率分析 22 5.2.4熱性質分析 22 5.3 mPEG-Chitosan水膠體薄膜分析 22 5.3.1 mPEG-Chitosan水膠體薄膜製備結果 22 5.3.2含水率與膨潤率測定 22 5.3.3 pH值測定 22 5.3.4表面微結構分析 22 5.3.5體外材料重量損失測試 22 5.4生物相容性分析 22 5.4.1細胞毒性試驗 22 5.4.2 細胞貼附實驗 22 5.4.3材料與細胞生長情形觀察 22 5.5動物實驗 22 5.6 組織切片染色 22 第六章 結論與未來展望 22 第七章 參考文獻 22

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