在2009年世界衛生組織已把蛇傷歸類於被忽視的熱帶疾病，世界各地每年將近540萬起蛇咬傷事件，並造成8萬至14萬的死亡人數，更有死亡數三倍的人們飽受截肢之苦，面臨永久性殘疾，因此具備有效用的抗蛇毒血清是必要的，在台灣每年約有1000筆蛇傷案例，其中眼鏡蛇因數量較多且與人們生活環境重疊，為常見咬傷案例的蛇種。
台灣眼鏡蛇毒主要毒性成分為心臟毒素、神經毒素及磷酸水解酶A2，先前有研究指出，現今市面上利用粗蛇毒做抗原，進行免疫所生產出的抗蛇毒血清，主要認含量少且較無毒性的高分子量蛋白，而相較之下，毒性較強蛋白部分，反而血清辨認能力較弱。同時研究指出，若單純利用心臟毒素、神經毒素及磷酸水解酶A2做抗原，生產出的血清會大幅改善此問題，並有效提升血清的效價。且若抗原中添加Scrambled CTXA3製造出的血清，會增加認心臟毒素的種類，因此我們團隊利用主成分為心臟毒素、神經毒素及磷酸水解酶A2的免疫原，生產出對粗蛇毒有更高中和效價的增效型血清及近一步在免疫成分加了Scrambled CTXA3的廣效型血清，在對各型的血清利用HPLC抗蛇毒血清分析技術、酵素免疫吸附法及小鼠實驗來進一步分析中和活性，並比較這些技術的優點。最後，透過ELISA和NMR滴定實驗研究神經毒素的抗原決定位。

The World Health Organization already classified snake injuries as a neglected tropical disease in 2009. Near 5.4 million snake bites occur every year in the world, causing 80,000 to 140,000 deaths and more than three times of people suffering from amputation and permanent disability. Thus, to prepare an effective antivenom is essential. There are about 1,000 cases of snake injuries each year in Taiwan. Among them, the injuries caused from Taiwan cobra is relatively common because the higher population and living area overlapping with human living environment. The main toxic components of cobra venom are cardiotoxin, neurotoxin and phospholipase A2. Literatures have reported that the current antivenom on the market mainly recognizes the high-molecular proteins in cobra venom, which is less toxic and with very low ratio. In contrast, the toxic part is less recognized. Here, we perform a study to manufacture new-type antivenom by only immunizing cardiotoxin, neurotoxin and phospholipase A2. The strategy improves the yield of antivenom production and meanwhile, effectively increases the serum titer. We also test another procedure by adding scrambled cardiotoxin A3 into the antigen to enhance the ability of serum to recognize all types of cardiotoxins. The different antivenom are produced by immunizing horses. The antigen containing only cardiotoxin, neurotoxin and phosphohydrolase A2 creates synergistic effect in neutralizing crude venom. The antigen further including scrambled CTXA3 therefore demonstrate broad effect. The serum of each preparation is analyzed by HPLC antivenom analysis method, ELISA and animal test to define the neutralization activity. We compare the advantages of the individual methods. In final, the epitope of neurotoxin is explored by ELISA and NMR titration experiments.

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