由於含微量元素之生物分子在生物體內扮演著許多重要的生化功能，相關的研究工作近年來業已成為分析化學領域中的一個重要的研究主題。但由於生物體內含有微量元素的生物分子通常係以極低濃度存在，且生物樣品的基質十分複雜，致使在分析方法之發展上仍有諸多困難尚待克服。
本研究研究的目的旨在建立一套二維形體篩分/逆相層析技術，並配合感應耦合電漿質譜法及電灑質譜法，進行口服液樣品及血清樣品中含金屬分子(metal-binding biomolecule)之鑑定工作。在評估所欲建立之分析系統對含有特定微量元素之生物分子鑑定能力的過程中，有鑑於文獻中有關金屬硫蛋白分子的研究已相當透徹，因此於方法建立之初，本研究係選擇金屬硫蛋白(metallothionein, MT)來進行方法可行性的研究。在確立所欲建立分析方法的可行性之後，本研究亦嘗試利用所建立之分析程序，試圖探求其在鑑定兔子血清中含有Cu，Zn，Co等元素之生物分子時可能遭遇之困難及適用性。
根據目前所獲致之實驗結果可知，本研究所開發之2D-SEC/RPLC層析系統，已可順利地鑑定出口服液樣品及兔子血清樣品中的金屬硫蛋白分子及維生素B12分子。但受到RPLC-ESI-MS偵測的靈敏度及RPLC分離效果的限制，目前仍無法確定地鑑定出血清樣品中含銅及鋅的生物分子。為克服上述分析技術上的限制，未來應可藉由提高濃縮倍數，增進RPLC分離純化效果及改用更為靈敏以及具有更寬mass range的分子鑑定技術來達成鑑定生物體內金屬蛋白的目的。

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