生物醫藥研究及應用已進入了分子的層級，相較於傳統生化方法所看到的是眾多分子的平均表現，單分子技術藉由即時的量測個別分子的空間解析即時間解析行為，為生物醫學提供了一創新研究的舞台。在奈米尺度下觀測單一分子的動態行為是十分複雜且困難的，因此本實驗室致力於發展高解析度雷射光鑷子系統，以提供一精密的單分子研究平台。
由於實驗室之前並無光鑷子系統相關經驗，開始將藉由簡化版雷射光鑷子系統的設計及建構，以建立系統相關經驗，並使簡化版系統表現達現行商用系統水準。為了以奈米空間解析度量測生物單分子，進而設計及建立高解析度雷射光鑷子系統。其空間解析度已達1nm，光鑷子剛性係數為0.025pN/nm (雷射功率50mW)，施力量測範圍可由數十fN至數十pN。另外，傳動素相關生物實驗將附於附錄。

The optical tweezers technique is wildly used to constrain and move small particles, like single cells and organelles, in solution by using a light microscope and a laser beam. The force generated by laser tweezers can stretch, bend, or distort single macromolecules, such as DNA and RNA. By providing an unprecedented means to manipulate on the microscopic scale, optical tweezers have played a significant role in biology, especially in the single molecular field.
In this thesis, we describe the design and construction of optical tweezers system. At the beginning, we establish the simplified optical tweezers system, which can be used in the cellular level research. In order to study the single motor protein’s behavior in the nanometer scale, we design and construct the high-resolution optical tweezers system. Currently, the high-resolution system can measure the nanometer displacement of the sample. The system’s stiffness is calculated by using the Lorenztian model with well alignment. The system can apply the force ranging from femtonewtons to nanonewtons. This is ideal range for exerting forces on biological molecule and for measuring their response.

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