Enterovirus 71 (EV71) is one of the major causes of hand-foot-and-mouth disease (HFMD) and sometimes a patient infected with EV71 may suffer from serious neurological complications even dead. It is hard to directly observe the causes that make the phenotype difference in simply genome comparison. For example, NCKU9822 and Tainan/6092/98 are very similar strains but NCKU9822 causes fatal outcome and the other does not. We suppose it should be satisfied many factors to cause fatal outcome.

To predict the pathological patterns, some have to get the polyprotein features and functions, some have to analyze the properties of different genotypes, and the other have to notice the years and areas of outbreaks. It is not easy to collect that information. For our research, we do the database EVdb to be a prototype for the enterovirus research to enhance the data from Genbank feature annotation, fill up pathologic phenotypes from collected literature and predict the polyprotein cut sites. Scientists can be faster to design a new examination data. We classify the pathological phenotypes into 3 degrees as following: (1) acute/fatal neurovirulence, such as EV71 genotype C2 and B4 fatal strains, (2) slightly neurovirulence, such as coxsackievirus A16, (3) non-neurovirulence, such as EV71 genotype B3.

In the result, the three pieces of genome of EV71 genotype B3, 3C, 3D,and 3'UTR that related with viruses replication are quite different with genotype B4 in the same genotype group B (accumulated distance >=0.61). However, It is much closer to the neighbor species coxsackievirus A16 with slightly neurovirulence (accumulated distance <0.38). In the other pieces of polyprotein, we cannot observe such difference. In conclusion, we suggest the effective of virus replication is one of the important factors to cause the fatal acute neurological complications.

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