本篇論文所呈現的層析晶片具有親疏水梯度的奈米碳管固定相，取代傳統液相層析中使用的多種組成分移動相梯度沖提，延流體行進方向上，奈米碳管表面有一親疏水梯度，待分離的樣品依自身的親疏水程度吸附在相對應的碳管表面，而達到分離的效果。藉由光化學反應和控制奈米碳管的紫外光照射量，可以達到親疏水程度的不同；樣品在管柱中分離後並不沖提出來，而是直接噴灑基質於晶片上方，直接進行質譜的測量。
質譜儀在生物高分子上的運用，已成為了解生命演化過程中不可或缺的重要工具。近年來，蛋白質體學已成為二十一世紀最熱門的研究主題，隨著愈來愈多的基因排序被解碼，研究人員現正探究基因密碼和不同蛋白質間的關係，進而了解致病的原因。由於最近質譜技術在生化科技方面的軟體（蛋白質序列的軟體）不斷地有突破性的發展，更有利於質譜學家及生化學家進行蛋白質體學的研究。

This thesis presents a study of capillary electrochromatography chip with hydrophobic gradient stationary phase consist of carbon nanotubes (MWCNTs) array of nano-channels. Driving by EOF (Electroosmotic flow),proteins entered the separation channel and were attracted by the modified MWCNTs surface and then separated according to their own characteristics.
A human cell contains about 20,000 kinds of proteins anytime. So far the 2D Electrophoresis is the most efficient separating approach. The advantage of this approach is that specific protein can be observed by controlling specific pH range. However, a large area of gel is needed for high resolution. Analyzing and matching through mass spectrometry require long time. Two types of ionization source are commonly used in proteomics, MALDIand ESI. ESI usually combine with chromatography as LC-MS, after separation the substance go through ionizer directly. MALDI will obtain the mass information. But limited by the mechanism, substances have to mixed and crystallized with matrix individually. When the components’ concentrations wildly differ from each other, the rare signal will be sheltered. However, MALDI has advantages like easy operation, auto equipment for producing protein, increase efficiency, high sensitivity, etc. If we can simplify the step of crystallization, we can obtain more information from MALDI.

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