研究生: |
邱偉倫 |
---|---|
論文名稱: |
綠水螅體內共生藻的親緣性分析 Phylogenetic analysis of the symbiotic Chlorella in green hydra |
指導教授: | 徐邦達 |
口試委員: | |
學位類別: |
碩士 Master |
系所名稱: |
生命科學暨醫學院 - 生物資訊與結構生物研究所 Institute of Bioinformatics and Structural Biology |
論文出版年: | 2009 |
畢業學年度: | 97 |
語文別: | 中文 |
論文頁數: | 61 |
中文關鍵詞: | 綠水螅 、共生藻 、親緣性分析 |
相關次數: | 點閱:1 下載:0 |
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摘要
雖然綠水螅與小球藻的共生現象在1970至1990年之間有許多的研究發表,但至今卻沒有發現有文獻資料針對綠水螅內共生藻作定序或親源性分析。在生理實驗上,我們主要方向在確認分離培養後的小球藻在大小、分裂數以及葉綠體分布上和水螅體內的小球藻之間的差異。同時與過去研究互相比較、驗證,但生理現象的觀察並無法直接判斷培養出來的小球藻品系。進一步的鑑定則是依靠PCR反應放大胞核及葉綠體SSU rDNA基因片段,並與基因資料庫進行比對,我們在目前基因資料庫中沒有發現完全符合的品系,但在親源性分析上,可以發現C. sorokiniana IAM C-210和C. pyrenoidosa IAM C-210比較接近我們分離培養出來的藻種。
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