細胞色素P450酵素是含有血紅素的一巨大家族，為第一期酵素參與催化內在物質或外來物質如藥物、環境的毒素以及食入物質的氧化還原等代謝作用。依據蛋白質序列的相似性，細胞色素P450酵素可以區分為各種不同的家族以及亞族。
在本實驗中利用RACE的方法，選殖出斑馬魚七種細胞色素酵素基因，經P450命名委員會命名，分別為CYP3A65、CYP1C1、CYP2AD3、CYP2K8、CYP2V1、CYP2X10以及CYP2X12。以全覆式定位雜交的方法，鑑定出CYP3A65基因在幼魚時主要表現在肝臟以及腸道。CYP1C1基因在胚胎期至孵化後均呈現在血管部位。CYP2K8基因在未孵化前主要是在鰓弓區域，而在孵化後則是在胰臟以及腸道表現較高，在鰓弓區域的表現降低。CYP2AD3 基因表現在軟腦脊膜、鰓弓、肝臟以及腸道，CYP2V1則表現在嗅球、鰓弓、肝臟以及腸道，CYP2X10以及CYP2X12則表現在腦部以及肝臟和腸道。
藥物敏感試驗，顯示固醇類的dexamethasone、巨環類抗生素rifampicin以及環境荷爾蒙2,3,7,8-tetrachloro-p-dioxin皆可增強CYP3A65在腸道中的表現。CYP1C1亦會受到dexamethasone和TCDD的誘發，在血管位置表現量明顯增加。
此外，以anti-sense morpholino來減弱AhR2的轉譯，會造成原生性以及由TCDD、dexamethasone或rifampicin在腸道所誘發的CYP3A65基因表現明顯受到抑制。相同的morpholino亦會對CYP1C1原生性以及TCDD或dexamethasone所誘發基因的表現產生抑制作用。由這些結果，顯示AhR/ARNT訊息通路對於調控這些藥物誘發CYP3A65以及CYP1C1基因的表現扮演著重要角色。

Cytochrome P450 is a superfamily of heme containing monooxygenase that catalyze the oxidative and reductive metabolism of many drugs, environmental chemicals, and endogenous compound. On the basis of sequence similarity, the CYPs can be classified into various families and subfamilies.
Here I have cloned seven CYP genes by rapid amplification of cDNA ends (RACE) approach. On the basis of nucleotide and amino acid sequences, the identified cDNAs was assigned as CYP3A65, CYP1C1, CYP2K8, CYP2AD3, CYP2V1, CYP2X10 and CYP2X12 by the Cytochrome P450 Nomenclature Committee. CYP genes expression pattern in zebrafish embryo was investigated by whole mount RNA in situ hybridization. The CYP3A65 mRNA is initially transcribed only in the liver and gut after hatching. In contrast the CYP1C1 mRNA is expresse in blood vessel. Weak transcription of CYP1C1 is still detectable in larval vascular system. The CYP2K8 mRNA is expressed in the branchial arch before hatching. After hatching the CYP2K8 is mainly expressed in the liver and gut. The CYP2AD3 mRNA is expressed in pia mater, branchial arch, liver and gut. The CYP2V1 mRNA is expressed in olfactory bulb, branchial arch, liver and gut. The CYP2X10 mRNA is expressed in the brain, liver and gut. The CYP2X12 mRNA is expressed in the brain, liver and gut.
During the larval stages, CYP 3A65 transcription in the gut is greatly enhanced by steroid dexamethasone, macrocyclic antibiotic rifampicin or TCDD exposure. CYP1C1 transcription is also enhanced by dexamethasone and TCDD treatment.
Repression of AhR2 translation by antisense morpholino oligonucleotides abrogated both of constitutive and TCDD, dexamethasone or rifampicin-stimulated CYP3A65 transcription in larval intestine. Similar abrogation of constitutive and drug-induced CYP1C1 transcription is also observed in AhR2-morpholino injected embryo.

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