本篇實驗欲藉由波長365nm的紫外光在光纖內全反射傳遞的同時，於光纖表面的另一端產生漸逝波，來誘導靠近感測表面的光誘導分子 鄰-硝基苯甲醛 (o-Nitrobenzaldehyde , o-NBA) ，使此分子於短時間(nanosecond)內進行光化學反應並釋出大量的氫離子，改變靠近感測表面的環境酸鹼值，將表面的抗體抗原配對分離，利用此機制建立出一免疫感測表面再生技術，來達到體內重覆檢測的效果。實驗結果顯示，經由電化學量測的實驗，距離表面100nm 高度的感測表面，局部環境酸鹼值在1分鐘內可由pH=7.0大幅下降至pH=2.7，3分鐘後酸鹼值降至2.1而趨於穩定，另外，隨著距表面高度的上升，溶液酸鹼值下降的幅度愈趨平緩，而在距離表面500μm之外的環境酸鹼值於5分鐘內幾乎維持恆定不受影響 (pH~7)。於平面感測系統上，本實驗機制的免疫分離效果於10分鐘內可以達到85%，相對於傳統利用酸洗的方法，不僅可以達到傳統上酸液分離抗體抗原的效果，並因其只在表面激發出氫離子，使得此機制能夠將酸液侷限於感測表面，不致於影響外圍環境酸鹼值恆定，應用於體內感測時可避免傳統酸洗直接加入酸液對活體所造成的傷害，另外，實驗結果顯示，感測表面重複接合效果良好，重複感測4次並不會造成一級抗原的活性喪失。光纖感測系統的實驗上，本研究初步完成光纖漸逝波系統的建置，利用側向偵測波長460nm螢光訊號的機制，經由光纖回傳訊號至光譜儀的實驗上，已確認可以利用光吸收曲線上波長460nm是否存在波峰，來觀察是否有效將感測區表面抗體抗原配對分離。
因此，本實驗欲藉由漸逝波誘導o-NBA分子釋出氫離子來將抗體抗原配對分離的機制與生醫微光纖檢測平台相互結合，來達成體內重覆檢測的願景。

In this paper, we propose a novel method employing photo-induced immune complexes dissociation for surface regeneration. Protons, released by UV irradiation on a photochemical molecule o-Nitrobenzaldehyde (o-NBA), were employed to rapidly elevate solution pH level from 7.0 to 2.7 within 60sec. Applying to the dissociation of antigen/antibody conjugation, the elution efficiency of 85% within 10 minutes can be approached. This method can effectively confine the proton generation region out of reaching most of the target protein molecules in the solution by confining the irradiation of UV light to minimize protein damage, which is easily encountered by traditional acid washing way. Therefore, a reusable immunoassay can be realized.

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