簡易檢索 / 詳目顯示

研究生: 林淑娟
Shu-chuan Lin
論文名稱: 以COI基因核酸序列分析右突新鏢水蚤 Neodiaptomus schmackeri 及錐肢蒙鏢水蚤Mongolodiaptomus birulai族群之遺傳變異
Population Variable of Neodiaptomus schmackeri &Mongolodiaptomus birulai in Taiwan as Inferred from COI Sequences
指導教授: 張立雪 博士
Dr. Li-Hsueh Chang
口試委員:
學位類別: 碩士
Master
系所名稱:
論文出版年: 2004
畢業學年度: 92
語文別: 中文
論文頁數: 63
中文關鍵詞: 哲水蚤粒線體基因交流族群分化
外文關鍵詞: Calanoida, mtDNA, COI, gene flow
相關次數: 點閱:2下載:0
分享至:
查詢本校圖書館目錄 查詢臺灣博碩士論文知識加值系統 勘誤回報
  • 本研究是以粒線體DNA之COI核酸序列當作分子遺傳標記,共分析台灣地區淡水產哲水蚤(Calanoida)右突新鏢水蚤 Neodiaptomus schmackeri 四個族群51個樣本,錐肢蒙鏢水蚤Mongolodiaptomus birulai 五個族群65個樣本,定序結果獲得右突新鏢水蚤Neodiaptomus schmackeri 635bp的序列長度,錐肢蒙鏢水蚤Mongolodiaptomus birulai 655bp的序列長度,分別得到8種及14種單套基因型(Haplotype)。以族群間分化指數(Fst)及基因交流指數(Nm)分析,除台南曾文水庫及烏山頭水庫的錐肢蒙鏢水蚤Mongolodiaptomus birulai彼此基因交流暢通外(Fst=0.058; Nm=4.04),其餘族群間基因交流皆不順暢(Fst:0.4~0.99; Nm:0~0.37),顯示族群分化程度明顯,隔離效應顯著。
    以近鄰歸群法(neighbor-joining method)分析所得之地理親緣關係樹(Geophylogenetic tree),其結果皆顯示新竹寶山水庫之族群與其他地區族群分歧較大,成為一獨立之類群,分支可信度為99%。同時,各地的族群皆各自成一類群未有相互混雜之情形,分化程度明顯但未符合距離隔離模式,各地族群大致會聚集在一起,唯一例外的是錐肢蒙鏢水蚤Mongolodiaptomus birulai中的曾文水庫與烏山頭水庫個體在圖形上會呈現鑲嵌狀,且有些基因型會同時出現在兩地,而無明顯的分化現象。


    I use the mtDNA COI ( Cytochrome Oxidase I )sequence as genetic maker to analysis the population genetic structure of two species freshwater copepod, Neodiaptomus schmackeri and Mongolodiaptomus birulai (Calanoida:copepoda) in Taiwan. I have four populations, 51 individuals of N. schmackeri, and five populations, 65 individuals of M. birulai. I compared nucleotide sequences of a 635 bp fragment from the COI gene of N. schmackeri, 8 unique haplotypes were recorded ; 655 bp fragment from the COI gene of M. birulai, 14 unique haplotypes were recorded. Except the gene flow between the Tseng-wen reservoir and Wu-sa-nto reservoir was umlimted (Fst=0.058; Nm=4.04), the gene flow of all the other populations are limited (Fst:0.4~0.99; Nm:0~0.37). Based on the COI gene diversification data, I suggest
    that the most population of N. schmackeri and M. birulai are isolated. The Geophylogenetic relationships are inferred by the neighbor-joining method. In the M. birulai, all the individuals are grouped in the right place of collecting site, but the Tseng-wen reservoir and Wu-sa-nto reservoir are grouped together. In the N. schmackeri, all the individuals are grouped in the right place of collecting site, the most dived population is N. schmackeri of Bao-shan reservoir with confidence of 99%. Genetic distance was not correlated with geographic distance, the genetic diversification pattern is not comparable to higher isolation of longer distance separation.

    摘要 1 壹、前言 3 貳、材料與方法 7   一、樣本採集與保存 7   二、粗 DNA的萃取 7   三、PRIMER的設計 8   四、PCR(POLYMERASE CHAIN REACTION)聚合脢連鎖反應 8   五、電泳及染色 9   六、MTDNA定序 10   七、序列分析 10   八、地理親緣關係樹(GEOPHYLOGENETIC TREE)建立 12 參、結果 13   一、樣本採集 13   二、基因序列 13   三、遺傳變異分析 15   四、遺傳結構分析 17   五、地理親緣關係樹建立 18 肆、討論 19 伍、參考文獻 25 陸、圖表 28 附錄 53 附圖 61

    Bohonak, A.J. and D.G. Jenkins, 2003. Ecological and evolutionary significance of dispersal by freshwater invertebrates, Ecology Letters, 6: 783–796
    Braga, E., R. Zardoya, A. Meyer and J. Yen, 1999. Mitochondrial and nuclear rRNA based copepod phylogeny with emphasis on the Euchaetidae (Calanoida). Mar Biol 133:79-90.
    Brussard, P.F., P.R. Ehrlich, D.D. Murphy, B.A. Wilcox and J. Wright, 1985. Genetic distances and the taxonomy of checkerspot butterflies (Nymphalidae:Nymphalinae). J. Kansas Entomol. Soc. 58:403-412.
    Bucklin, A., and B.W. Frost, 2002. Molecules versus morphology:A comparative view of evolution within and among calanoid copepod species. In: 8th International Conference On Copepoda, National Taiwan Ocean University, Abstract Book p23.
    Bucklin, A., B.W. Frost and T.D. Kocher, 1995. Molecular systematics of six Calanus and three Metridia species (Calanoida: Copepoda). Mar Biol 121:655-664.
    Bucklin, A., S. Kaartvedt, M. Guarnieri and U. Goswami, 2000. Population genetics of drifting (Calanus spp.) and resident (Acartia clausi) plankton in Norwegian fjords. J Plankton Res 22:1237-1251.
    Bucklin, A. and P.H. Wiebe, 1998. Low mitochondrial diversity and small effective population sizes of the copepods Calanus finmarchicus and Nannocalanus minor: possible impact of climatic variation during recent glaciation. J Heredity 89:383-392.
    Collins, M.M., H.B. Britten, V. Rivers, 1993. Allozyme analysis of a known hybrid zone between Hyalophora euryalus and H. Columbia gloveri (Lepidoptera: Saturniidae) in the California Sierra Nevada. J. Res. Lepid. 32:79-88
    Darwin, C., 1859. The Origin of Species, Oxford University Press:London, pp439.
    Edmands, S., 2002. Phylogeocgraphy and the effects of postglacial expansion in the Noth American harpacticoid copepod Tigriopus Californicus. In: 8th International Conference On Copepoda, National Taiwan Ocean University,
    Abstract Book p43.
    Eyun, S.I., H.Y. Soh, H.L. Suh, and Y.H.Lee, 2002. Molecular phylogeny of the Korean Pseudodiaptomid Copepods (Calanoida):An assessment based on COI sequences. In: 8th International Conference On Copepoda, National Taiwan
    Ocean University, Abstract Book p45.
    Fu, Y.X. and W.H. Li, 1993. Statistical tests of neutrality of mutations. Genetics 133: 693-709.
    Gerken, S., G. Wyngaard, and C.E.F. Rocha, 2002. A molecular phylogeny of the cyclopinae, with a comment on possible relevance of chromation diminution on rates of speciation. In: 8th International Conference On Copepoda, National Taiwan Ocean University, Abstract Book p47.
    Grbbert, S., and H.U. Dahms, 2002. Phylogeny of calanoid copepods from the Antarctic. In: 8th International Conference On Copepoda, National Taiwan Ocean University, Abstract Book p53.
    Haeckel, E., 1866. Generelle Morphologie der Organismen, II. Berlin: Georg Reiner.
    Hanski, I., 1999. Metapopulation Ecology, Oxford University Press, Oxford, UK. Hillis, D. M. and C. Moritz (eds.) 1990. Molecular Sysematics, Sinauer Associates, Sunderland, Massachusetts.
    Hudson, R. R., M. Slatkin and W. P. Maddison,1992. Estimation of levels of gene flow from DNA sequence data. Genetics 132: 583-589.
    Levins, R., 1969. Some demographic and genetic consequences of environmental heterogeneity for biological control. Bull. Ent. Soc. Am., 15, 237–240.
    Linnaeus, C., 1758. Systema Naturae per Regna Tria Naturae, Secundum Classes, Ordines, Genera, Species Cum Characteribus, Differentiis, Synonymis, Locis. Editio Decima, Reformata, Tom I. Laurentii Salvii, Holmiae.
    Machida, R. J., M.U. Mmiya, M. Nishida, and S. Nishida, 2002. Complete mitochondrial DNA sequence of (Crustacea: Copepoda), Marine Biotechnology 4: 406-417.
    Nei, M., 1987. Molecular Evolutionary Genetics. Columbia Univ. Press, New York.
    Nei, M., 1996. Phylogenetic analysis in molecular evolutionary genetics, Annu. Rev. Genet. 30:371–403.
    Saitou, N. and M. Nei, 1987. Neighbor-joining method, Mol. Biol. Evol. 4 , 406-425
    Schizas, N.V., B.C. Coull, G.T. Chandler and J.M. Quattro, 2002. Sympatry of distinct mitochondrial DNA lineages in a copepod inhabiting estuarine creeks in the southeastern USA, Mar. Bio. 140: 585–594.
    Shaklee, J.B., C.S. Tamaru, R.S. Waples, 1982. Speciation and marine fishes studied by the electrophretic anaiysis of protein, Pacif. Sci. 36: 141-157
    Tajima, F., 1989. Statistical method for testing the neutral mutation hypothesis by DNA polymorphism. Genetics 123: 585-595.
    Thrope, J. P., 1983. Enzyme Variation, Genetic Distance and Evolutionary Divergence in Relation to Levels of Taxonomic Separation. In G. S. Oxford and D. Rollinson [ed.] Protein polymorphism: adaptive and taxonomic significance. Academic Press, New York, NY.
    Wright, S., 1978. Evolution and Genetics of Populations, vol. 4. Variability Within and Among Natural Populations. University of Chicago Press, Chicago, IL.
    何孟娟 2000. 大琉璃紋鳳蝶與琉璃紋鳳蝶親緣關係之探討,國立台灣師範大學生物研究所碩士論文, pp73頁.
    何瓊紋 2001. 粒線體 COI 基因之歧異顯示了台灣條紋章魚同胞種的存在,國立臺灣大學海洋研究所碩士論文, pp57頁.
    余正平 2002. 台灣及其鄰近地區養殖牡蠣(Crassostrea spp.)族群遺傳變異之研究, 國立中山大學海洋生物研究所碩士論文, pp53頁.
    李峻瑋 2001. 以粒線體之細胞色素氧化脢I部分序列探討臺灣地區人面蜘蛛(Nephila maculata)之族群遺傳結構,東海大學生物研究所碩士論文, pp67頁.
    沈嘉瑞主編 1979.中國動物志 節肢動物門 甲殼綱 淡水橈足類,科學出版社, pp450頁.
    邵廣昭 2003. 分子生物技術在動物系統分類上之應用,分子生物技術在生物分類上之應用研習會講義http://life.nthu.edu.tw/~labtcs/ls5105/
    邱郁文 2002. 田螺形態與遺傳變異之研究, 國立臺灣大學動物學研究所碩士論文, pp114頁.
    姜鈴 1990. 應用粒線體DNA分析台灣的九孔族群關係,國立台灣大學漁業科學研究所碩士論文, pp103頁.
    施心展 1999. 四種青蟹(Scylla spp.)在外部形態上及核糖體DNA上之差異,國立中山大學海洋生物研究所碩士論文,pp56頁.
    郭瓊華 2002. 台灣蜓蜥族群遺傳結構之研究,國立台灣師範大學生物研究所碩士論文, pp74頁.
    陳怡安 2001. 應用粒線體DNA探討Doratosepion (Cephalopoda:Sepiidae)之分類地位及烏賊屬之演化情形,彰化師範大學生物研究所碩士論文, pp98頁.
    陳嘉芬編著 2002. 現代遺傳學Ⅱ, 藝軒圖書出版社, pp704頁.
    黃鈞漢 2003. 以mtDNA D-loop片段探討艾氏樹蛙(溪頭地區)之族群遺傳結構,彰化師範大學生物研究所碩士論文, pp64頁.
    葉昌憲 1994. 台灣水鹿粒線體DNA序列之分析,國立台灣師範大學生物研究所碩士論文, pp44頁.
    劉健合 2000. 台灣地區烏魚粒線體DNA控制區序列變異及族群遺傳之研究,國立台灣大學動物學研究所碩士論文, pp59頁.
    魏群、崔麗華、楊淑杰、馬晴、姜國華、向本瓊、劉玉編著 2001.分子生物學實驗手冊,九州圖書文物有限公司,pp234頁.

    無法下載圖示 全文公開日期 本全文未授權公開 (校內網路)
    全文公開日期 本全文未授權公開 (校外網路)

    QR CODE