由於科技的進步與快速發展，醫療技術品質也隨之提高，人們對疾病的研究與探索也愈來愈全面化。在過去生物微流體晶片方面的研究，大多都是利用二維平面化的細胞培養為基礎來進行其他生物臨床實驗如藥物測試等，但是實際上人體內組織是由三維立體的結構所組成，故這些生物測試的實驗結果會與實際上人體內的情況有出入。為了創造仿生微環境，微流體的研究結合了組織工程來模仿人體內真實的三維組織結構，並開發出具有整合系統能力的微流體生物晶片，此工程與技術的結合締造出一個能夠供於生物實驗且具有三維組織結構的微系統晶片。本研究利用流體力學的層流現象與具有生物相容性的多孔隙光固化水膠材料：Gelatin Methacrylate (GelMA) 作為細胞排列與固定封裝的方法，並搭配三維微流道的設計強化層流現象的表現，最後並以此水膠材料作為細胞排列後能夠固定封裝的媒介。在此研究，我們利用層流現象來建構一個三維的細胞排列，藉由兩種細胞的排列在微流體晶片形成肝臟組織結構，預期在晶片上呈現仿生的肝臟組織。此外，我們使用HepG2肝癌細胞以及3T3纖維母細胞來進行層流排列，模仿體內之肝細胞及內皮細胞並做三維排列共培養，並且透過微流體技術模擬肝臟內之血流流向，在體外建立一個具有仿生功能的肝臟組織結構，作為未來生物臨床實驗的研究平台。本研究利用流體力學層流現象初步建構之三維肝組織結構，其三維肝臟組織結構高度約為80~93μm，經由我們的研究期待在未來的研究上能夠有前瞻性的發展。

Due to the improvement of technology and the rapid development on our society, the quality of medicine has been enhanced. The research and exploration of people's diseases are also more comprehensive. In the past, majority of the microfluidic biochip applications based on two-dimensional planar cell culture are for biologically clinical trials such as drug testing. However, the human body tissue is composed of three-dimensional structure. Thus, the past biological results might differ from actual circumstances in the human body. In order to establish bio-mimic environments, we combined the microfluidics with the tissue engineering to approach the three-dimensional tissue structure mimicking that in the human body. We developed a micro-system chip with three-dimensional structures for targeting biological experiments. In this study, laminar flow phenomena and biocompatible porous photocurable gelatin materials were used for cell patternings and encapsulations. Gelatin Methacrylate (GelMA) was an intermediary for encapsulations and was used to fortify the cell-cell interactions after cells were patterned. We used laminar flow to set up three-dimensional patterns of two types of cells to form our bio-mimic liver tissue on the three-dimensional microfluidic chips. HepG2 cells and 3T3 fibroblast were used here. Moreover, we simulated the flow of blood through the microfluidic techniques to construct in vitro biomimetic function of the liver tissue structure. In this study, the three-dimensional structure of the liver tissue is initially patterned by the laminar flow phenomenon. The three-dimensional structure of the liver tissue is approximately 80 to 93 μm in height, which can be developed prospectively through our research in the future.

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