酸鹼度與鹽度對抗菌胜肽功能影響｜國立清華大學博碩士論文庫

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研究生：	葉伯壽 Yip, Bak-Sau
論文名稱：	酸鹼度與鹽度對抗菌胜肽功能影響 Effects of pH and Salinity on the Antimicrobial Peptides
指導教授：	程家維
口試委員:
學位類別：	博士 Doctor
系所名稱：	生命科學暨醫學院 - 生命科學系 Department of Life Sciences
論文出版年：	2009
畢業學年度：	97
語文別：	英文
論文頁數：	59
中文關鍵詞：	抗菌胜肽、酸鹼度、鹽份濃度
外文關鍵詞：	antimicrobial peptide, pH value, salinity
相關次數：	點閱：3 下載：0
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隨著微生物對抗生素藥物的抗藥性發展，抗菌性胜肽的開發及研究，是一項重要而嚴肅的課題。從許多生物天生的防禦機制中發現的抗菌胜肽對細菌與黴菌的抑制作用，提供了一個有效而新穎的方式。然而抗菌胜肽處於不同酸鹼度及不同鹽度的環境對微生物的抗菌效果，會影響抗菌性胜肽的殺菌效果,限制臨床使用的可行性。本研究是針對序列為Ac-KWRRWVRWI-NH2富含色氨酸 (Trp-rich) 的胜肽Pac-525，D-Nal-Pac-525及另一富含組氨酸的胜肽 P113 (AKRHHGYKRKFH - NH2 ),對格蘭氏陽性(Gram positive)，格蘭氏陰性(Gram negative)細菌及真菌(fungus)的抗菌效用進行研究。本研究使用核磁共振儀來測定P-113結合擬態的膜ＳＤＳ微膜 micelles□之結構，同時利用螢光光譜儀與圓二色譜來探討抗菌胜肽在膜環境的特性與穿透能力。本研究結果發現酸鹼度及不同鹽份濃度對P-113及Pac-525的效能有嚴重影响，但酸鹼度及不同鹽份濃度對D-Nal的影響有限。

Abstract                                                     I
中文摘要                                                    II
Chapter 1  Introduction                                   1
Chapter 2  Anti-yeast Activities of a Novel D-β- naphthyl- alanine Substituted Antimicrobial Peptide Are Independent from Salt                                                     4
ABSTRACT                                                      4
INTRODUCTION                                            5
MATERIALS AND METHODS                                   6
RESULTS AND DISCUSSION                                   7
REFERENCES                                            9
Chapter 3 The pH and Salinity effects of Anti-bacterial Activity on Novel Tryptophan-Rich Peptide D-ß-naphthylalanine-Pac-525 , Pac-525 and P113                17
ABSTRACT                                                    17
INTRODUCTION                                           17
MATERIALS AND METHODS                                  19
RESULTS                                                    23
DISCUSSION                                           25
Chapter 4.  Solution Structure and Model Membrane Interactions of P-113, a Clinically Active Antimicrobial Peptide Derived from Human Saliva                        38
ABSTRACT                                                    38
INTRODUCTION                                          39
METHOD                                                   41
RESULTS                                                   43
DISCUSSION                                          46
REFERENCES                                          48

                                

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