熱治療在腫瘤治療上除了作為輔助療法，其優點也逐漸被重視並持續發展。本研究利用超順磁性奈米粒子以及交替磁場於腫瘤局部進行熱治療，並將放射治療與熱治療進行結合，探討腫瘤微環境的改變。結果顯示，熱治療的確具有抑制腫瘤生長的效果，增加腫瘤壞死面積、促使腫瘤巨噬細胞(Tumor associated macrophage)及表現Gr-1細胞表面抗原的嗜中性白血球細胞浸潤。搭配放射治療時抑制腫瘤效果更好，亦可對血管分布及型態上個別有破壞的效果。由上述結果可得知，熱治療不論單獨使用或作為輔助療法皆可改變腫瘤微環境，並促使免疫細胞浸潤。未來希望能利用腫瘤巨噬細胞攜帶超順磁性奈米粒子進入腫瘤中，利用核磁共振進行影像照影以及利用交替磁場給予熱治療，達到檢測及治療的效果，因此利用RAW264.7、BV2及小鼠腹腔巨噬細胞探討其吞噬能力及奈米粒子滯留於細胞內時間，以及缺氧環境對巨噬細胞吞噬能力的影響。實驗結果顯示，上述細胞皆具有吞噬能力，並且奈米粒子存在於細胞內的時間可長達19個小時以上，至於缺氧環境則可增進細胞的吞噬能力。

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