研究生: |
林光華 Lin Kuang-Hua |
---|---|
論文名稱: |
陣列式微流體生醫檢測晶片之研製 A Microfluidic Network Chip for Medical Diagnostics |
指導教授: |
錢景常博士
曾繁根博士 |
口試委員: | |
學位類別: |
碩士 Master |
系所名稱: |
工學院 - 動力機械工程學系 Department of Power Mechanical Engineering |
論文出版年: | 2002 |
畢業學年度: | 90 |
語文別: | 中文 |
論文頁數: | 96 |
中文關鍵詞: | 微型感測器 、生醫檢測晶片 、酵素電化學量測 |
相關次數: | 點閱:2 下載:0 |
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本文之研究主要是設計出一個可同時快速檢測血液中不同物質濃度之微型生醫檢測晶片。 本晶片主要可以分為兩個部分,一個是晶片之微流體系統的設計,另一個是微型感測器的部分。晶片之微流體系統設計的目的是使晶片能夠同時量測同一種檢體中不同物質的含量,而且不會使各種檢驗試劑混合在一起而有產生污染的情況,微流體系統的設計是採用SU-8光阻作為填充試劑的流道,以PDMS材質作為感測器的保護層以及血液的流道。 經由電漿處理將SU-8和PDMS微流道作親水處理後,可以藉由表面張力來填充試劑和運送血液檢體,而不需額外的驅動裝置。 藉由經由設計過的填充流道結構,試劑可以很快速、均勻、定量地被填充至感測位置。 在微型感測器的部分,本研究採用血醣感測器來證實所設計的多重檢測晶片的結構能在實際的環境中作檢測。 感測器依感測面積的大小,分別設計了兩種尺寸,分別為0.57 mm2和0.2 mm2。 血醣感測器採用酵素電化學量測的方式,測試結果得到感測面積較大的血醣感測器的靈敏度為18.58 nA/mM,感測面積較小的血醣感測器的靈敏度為5.42 nA/mM且量測之電流訊號與葡萄糖濃度呈現線性的關係。 因此本研究的所設計的微型生醫檢測晶片可以確實應用於同時對血液中不同成分濃度作檢測。
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