成人心肌組織缺乏再生能力，梗塞壞死的部位會被纖維組織所取代，嚴重影響心臟功能。本實驗室過去曾利用去細胞牛心包膜組織工程支架，結合多層間葉幹細胞片以做為心肌補綴片，並用來修補壞死的心肌組織。然而在植入體內後，由於短時間補綴片內部的血管新生程度不足，使得部分幹細胞會因為無法獲得充分的氧氣與養分而死亡，嚴重限制其治療效果。為了改善補綴片內的血管新生情形，本論文將含有人類臍帶靜脈內皮細胞(human umbilical vein endothelial cell, HUVEC)與臍帶血間葉幹細胞(cord-blood mesenchymal stem cell, cbMSC)的細胞片夾附至前述之多孔性去細胞牛心包膜支架內，以加快移植後補綴片內部的血管新生與組織再生。在體外實驗中，我們建立含有HUVEC/cbMSC細胞片後，藉由分析血管新生基因的表現量，找出最佳的HUVEC/cbMSC比例。免疫螢光染色結果顯示，細胞片可保存大量的細胞外間質，且部分cbMSC已開始表現平滑肌細胞(smooth muscle cell)或是周細胞(pericyte)的結構蛋白。將HUVEC/cbMSC細胞片夾附至多孔性去細胞牛心包膜支架內部以建立心肌補綴片後，可發現隨著體外培養時間的推進，培養基內的生長因子濃度有逐漸上升的趨勢。體內實驗部份，我們將上述之補綴片植入裸鼠背部的皮下組織，並以表面種有細胞之補綴片與不含細胞之補綴片做為控制組，於手術後第14天進行正子造影與取樣病理分析。實驗結果顯示，夾附至補綴片內部的細胞片可促進宿主細胞遷入支架內層，並刺激支架周邊與內部的血管新生。此外，我們亦發現部分人類細胞可被整合至宿主的血管中，直接參與血管的生成。以上實驗結果顯示，將HUVEC/cbMSC細胞片夾附至補綴片內部，可有效促進移植後補綴片內部的血管新生，改善支架內部細胞的存活情形。

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