如果說DNA編碼的基因組是生命的藍圖，那麼生物發育各階段中出現的各式圖樣生成系統則為其一系列生命工程圖集。這些工程圖引導、協調與控制生物系統中各個結構單元的遷徙與定位，並因而發展出其各自該有的功能。本論文旨在研究生物圖樣系統產生的過程如何控制生物的型態，以及生物型態如何回饋生物圖樣生成系統以形塑出各式生物圖樣。儘管發育中的細胞與組織的型態（即其形狀與大小）為前一發育階段的生物圖樣所決定，每一發育階段的型態亦能決定下一階段的圖樣生成。在此，單細胞生物如大腸桿菌，因其在細胞組織的簡潔特性而被選為研究圖樣生成動力學與型態控制的相互關係，而單細胞與單分子實驗方法則為本論文所採用，以研究將生命藍圖中的設計轉換為物理與化學環境中各種分子執行機構的運作生物物理原則。反應─擴散機制與化學波動現象為驅動生物分子在介觀世界中自組織與圖樣生成行為的要件，而為彰顯現本研究的科學底蘊，本論文依序呈現三種樣態結構：(1) 圖樣生成動力學；(2) 圖樣生成動力學與細胞型態；(3) 細胞型態控制。大腸桿菌的小細胞圖樣生成系統 (Min-protein patterning system)則作為核心研究對象並貫穿及橋接於論文內不同的研究主題與各個樣態結構中；本論文使用了不同科學領域多樣的科學語彙來傳達此一科研領域最新且重要的學理概念，最後，整體的研究成果則以作者對細菌生物物理學發展前沿的心得與獨特觀點作為總結。

If the DNA-coded genome is the blueprint for life, then pattern-forming systems in series of developmental stages are the collection of construction maps to instruct, orchestrate and control every structural components in an organism to migrate, localize and function as what they should be. The dissertation presented here is intended to investigate how biological patterning realizes cell morphology control and how cell morphology feedbacks to biological patterning. In developing tissues, the shape and size (morphology) in every developmental stage also determines next-stage pattern formation, even though the morphology of cells and tissues are determined from preceding-stage patterns. Herein, single-cell organisms such as bacterium Escherichia coli was chosen for its simplicity in cellular organization to investigate the interconnections between patterning dynamics and morphology control. Single-cell and single-molecule approaches were adopted to investigate biophysical principles that transform designs in the blueprint into executable machineries in the physical and chemical contexts. Reaction-Diffusion mechanism and chemical wave phenomenon are the very essence to drive biomolecules self-organization and patterning in mesoscopic world. As following the research paths toward scientific merits, the dissertation are organized to present three movements: (1) Patterning Dynamics; (2) Patterning Dynamics and Cell Morphology; and (3) Cell Morphology Control. Min-protein patterning system in E. coli is the kernel subject to bridge different research topics among these movements. The scientific language spoken in the dissertation is multi-dialectal in different disciplines such as to interpret new and key concepts in the field. These research efforts are concluded in author’s perspectives on the frontier of bacterial biophysics.

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