本研究針對奈米光學免疫感測晶片的研究，可應用於醫學、生化相關的檢測，利用特殊的奈米結構，可以讓訊號增強，達到快速篩檢的功用。本研究內容包含奈微米金陣列結構製作技術、化學合成粒子表面結構技術、表面化學修飾技術以及螢光訊號與拉曼訊號測試技術等。
生物檢測晶片已經研究多年，但是要達到少量的檢體又能快速並且明顯的得到高照訊比的目標一直是這方面研究的課題，近年來有許多人在研究特殊的奈米結構用以增強拉曼訊號，若能夠利用上述之方法，將可以減少檢驗物質並且提高訊號。而前述的奈米光學免疫感測晶片，是利用金屬粒子表面修飾一層自組裝單分子膜，再用單分子膜上面官能基與抗體官能基鍵結，抗體與抗原之間有互相辨認的特性，達到感測的機制。
為了滿足量少又能使訊號增強的需求，本研究設計了一種三維奈米等級的粗糙表面結構，搭配不同參數條件，可有效增加其訊號，由拉曼光譜檢測結果亦可獲得驗證。利用腺病毒當我們檢測的檢體，在結果方面，利用濃度107/μl的病毒溶液，有結構拉曼訊號增加為沒有結構的五倍，因此，透過此技術，不但有效減少檢體的量，並且有助於拉曼訊號之提升。

Abstract
In this study, optical immune sensor chip can be used in medicine, biochemistry related to the detection, the use of special nano-arrays, allowing the signal enhancement, to achieve rapid screening function. This study includes nano-micron manufacturing technology of gold array structure, chemical synthesis of particles, surface chemical modification techniques and fluorescence signals and Raman signal testing technology.
Biological detection chip has been studied for many years, but a small number of samples to achieve can be fast and clear signal that we need research in this area has been subject in recent years, many people in the study of nanostructures for specific enhanced Raman signal, if able to use the above method, can reduce the test material and to increase the signal. The aforementioned nano optical immune sensor chip is a layer of surface modification by metal particles self-assemble monolayer, monolayer and then the above functional groups and the antibody binding functional group, each between antibody and antigen identification features achieve sensing mechanism.
In order to increased the signal and less target in our chip, the study design was a three-dimensional rough surface structure level, with different parameters, which can effectively increase the signal detected by the Raman spectroscopy results can be verified, Therefore, through this technology, not only effectively reduce the amount of samples, and help enhance the Raman signals.

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