本研究主要以介電濕潤(Electrowetting on dielectric, EWOD)數位微流體晶片做為體外受精胚胎及培養平台，在油浴環境中以電濕潤力操控液珠之功能進行精子微液珠與卵子微液珠之移動與結合，模仿胚胎體內動態之環境，並探討介電濕潤晶片應用於生殖醫學之可行性。
精與卵微液珠滴於油浴介電濕潤晶片，以電極驅動精子與卵液滴之移動與結合。介電濕潤數位微流體平台放置於溫控式活體顯微鏡系統中維持適當細胞成長溫度與氣體濃度，模仿胚胎體內成長之環境，並以高解析度之即時觀測顯微鏡監測精卵結合與細胞成長之情形。實驗分為兩組，靜態培養組及使用介電濕潤晶片混合受精動態培養組。靜態胚培養將卵子液珠與精子液珠手動混合後靜置於油浴介電濕潤晶片；而動態培養組則是利用介電濕潤電極操控其上之精子與卵子微液珠混合受精，並每30分鐘移動受精卵液珠並持續一天，以模擬受精卵由輸卵管中移動至子宮著床之動態過程。
實驗結果發現介電濕潤晶片之動態培養與靜態培養組之精卵胚胎受精機率與細胞發育型態類似，此結果可證實介電濕潤數位微流體系統應用於生殖醫學之精卵結合的可行性。

This paper is to discuss a novel EWOD chip and mouse embryos cultured IVF (in vitro fertilization) as a EWOD chip. EWOD platform placed in temperature-controlled intravital microscopy system and provides temperature and gas concentration required for cell growth, in real time, high resolution microscope observation monitoring the situation of cell growth.

They were divided into two groups, static culture group and dynamic culture group. In dynamic culture group, EWOD chip for oocyte and sperm fertilization combined droplets, moving and observation.The Dynamic group step 1 is to draw HTF medium containing 0.08% pluronic and the drop in the dielectric and humid electrodes on the chip and cover oil. Step 2 is to use dielectric wafer wet sperm droplet beads mixed with sperm in the fluid motion. Step 3 the droplet moved on five EWOD electrodes dynamic culture. The period of each move will take 30 minutes, and every move will be finished within 30 seconds by using the operating voltage of 60V square wave frequency of 500 Hz.

Experimental results show the dynamic culture and static culture group, embryos fertilized embryos similar probability. The results confirm that EWOD systems can be used on fertilized embryos feasibility of reproductive medicine.

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