本研究發展出一套能有系統且簡便地將氧化石墨烯(GO)與銀奈米粒子(AgNP)結合，形成複合式奈米結構的合成與鑑定方法。利用電子穿隧顯微鏡、界達電位分析、電噴灑式即時分析和氣溶膠質量分析技術，可以測定出材料特性，包括物理大小、數量濃度、膠體穩定性、表面電荷、質量和組成。結果顯示複合式奈米粒子可以成功地被合成，並且可以藉由調整AgNP和GO的數量濃度比例，來達到控制大小(mobility size)、粒徑分佈、複合粒子之質量和組成的目的。結果顯示複合式奈米粒子的質量與其粒徑大小呈現正相關的關係，由此可知AgNP在每個複合材料上的數量與dp,m也是呈現正相關的關係。而複合式奈米粒子在膠體中的穩定度可以藉由改變dp,m與界達電位來做評估。當複合式奈米粒子的界達電位在10 mV以下或是 (define as│(IEP + pKa)/2 – pH│) < 1.75則會發生快速聚集的現象。此研究提供了一個有系統地合成GO-AgNP與鑑定性質方法之概念，在生醫發展的應用上有相當的潛力。

We report a facile approach for the controlled synthesis and characterization graphene oxide (GO)-silver nanoparticle (AgNP) hybrid nanostructure. Materials properties, including physical size, number concentration, colloidal stability, surface charge, mass, and conformation of GO-AgNP, were characterized successfully by a combination of electrospray-differential mobility analysis, aerosol particle mass analyzer, transmission electron microscopy, and zeta potential analysis. The results show that the nanohybrids were successfully fabricated. The mobility size, size distributions, mass of nanohybrids and the number of AgNPs and GOs per hybrid were tunable by choosing the number concentration ratios of AgNPs to GOs. The mass of nanohybrids was found to be proportional to mobility size of nanohybrids, indicating that the number of AgNP per hybrid is proportional to dp,m. Colloidal stability of nanohybrids was evaluated through the change of dp,m, and absolute zeta potential. Fast agglomeration was found when the absolute zeta potential of nanohybrids was below 10 mV and /or  (defined as |(IEP + pKa)/2 – pH|) < 1.75. The work presented here provides a proof of concept for systematically synthesizing of GO-AgNP, with the ability in characterization, which has shown to be promising for biomedical applications.

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