黑色素的含量及分佈狀況是決定皮膚及頭髮顏色的主要原因，由於酪胺酸酶是黑色素形成的主要關鍵酵素，所以利用抑制酪胺酸酶活性去阻止黑色素過度形成是目前最安全有效的美白方式。本實驗是使用chlorogenic acid和hydrobenzyl alcohols測試抑制酵素活性的作用，分別利用不同美白測試系統，探討活性成分對於黑色素生成及細胞生理機制的關係。結果顯示0.8 mM chlorogenic acid會使黑色素細胞中的黑色素含量降低56%，而4-hydrobenzyl alcohol濃度達1.0 mM時黑色素含量降低至45%，而且沒有觀察到細胞毒殺性的情形。此外由於在哺乳動物皮膚中黑色素的生成會受到角質細胞、纖維母細胞及其他調控因子的影響，因此我們利用黑色素細胞及角質細胞建立共同培養的系統作為皮膚模擬模式，來研究黑色素抑制及刺激因子在此系統中對於黑色素調控的影響。由實驗結果證實1.0 mM 4-hydrobenzyl alcohol在黑色素細胞及角質細胞建立的系統中黑色素含量降低33%，而且不會影響與黑色素形成相關的基因。在老鼠實驗中可以明確觀察到hydrobenzyl alcohols的美白功效性及安全性，而且在模擬皮膚的系統上也可以看到相同的結果。所以hydrobenzyl alcohols在美白保養品上的使用上有廣泛的應用價值，值得更深入研究及探討。我們也希望藉由模擬皮膚的系統能夠取代傳統動物實驗的方法。

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