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研究生: 徐維廷
Wei-Ting Hsu
論文名稱: 以樹枝狀高分子作為多功能藥物載體之應用
指導教授: 李育德
Yu-Der Lee
口試委員:
學位類別: 碩士
Master
系所名稱: 工學院 - 化學工程學系
Department of Chemical Engineering
論文出版年: 2007
畢業學年度: 95
語文別: 中文
論文頁數: 71
中文關鍵詞: 樹枝狀高分子藥物傳輸磁振造影
外文關鍵詞: dendrimer, drug delivery, MRI
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  • 本研究是以樹枝狀高分子為核心,樹枝狀高分子為一新發展的高分子,其特色在於化學鍵從中心的核由內向外發散,形成樹枝狀的分枝,利用其高度分枝的結構,在表面官能基上,可經由適當改質而接上不同的分子作多功能的運用,而將之應用在生醫材料方面。
    本實驗以PEG作為核心,採發散式合成法向外產生分枝的OH表面官能基,合成出一至三代的樹枝狀高分子,並在高分子表面接枝上經螯合穩定之造影活性釓元素MRI顯影劑、具癌症主動標的功能的葉酸、及抗癌藥物MTX。實驗結果顯示,由樹枝狀高分子所攜帶多個顯影劑,能大幅提高每單位分子造影劑的訊號強度,可有效運用於醫學造影上。而接上葉酸後,由於其癌組織辨識的效果,可增加載體被癌症細胞攝取的機會,因此在醫學上,未來可結合醫學造影以及癌症治療,利用此多功能藥物載體,一方面將藥物有效送至癌組織,一方面利用MRI隨時偵測治療情形。且材料經由細胞毒性測試,證實其具有生物相容性,因此在生醫材料的利用上,有其發展的可行性。


    摘要 謝誌 目錄 圖目錄 表目錄 表目錄 第一章 緒論 第二章 文獻回顧 2-1 樹枝狀高分子簡介 2-1-1 Dendrimer的起源與發展 2-1-2 Dendrimer的結構與特性 2-1-3 Dendrimer的合成方法 2-1-4 Dendrimer的應用 2-2癌症標的及藥物傳遞 2-2-1葉酸簡介 2-2-2抗癌藥物MTX簡介 2-2-3藥物傳遞系統 2-2-4 Dendrimer於藥物傳遞的應用 2-3磁振造影 2-3-1磁振造影簡介 2-3-2釓類MRI顯影劑及其改良方法 第三章 研究目的及動機 第四章 實驗部分 4-1 實驗藥品 4-2 實驗儀器 4-3 實驗流程 4-4 實驗步驟 4-4-1合成不同代數的PEG-core dendrimer 4-4-2合成PEG-core樹枝狀高分子顯影劑 4-4-3合成末端接枝葉酸及藥物之dendrimer 4-4-4 體外細胞實驗(in vitro biological test) 第五章 結果與討論 5-1樹枝狀高分子合成 5-1-1 1H-NMR結構鑑定 5-1-2 IR結構鑑定 5-1-3 純度鑑定 5-2 PEG-core樹枝狀高分子顯影劑 5-2-1 dendrimer鍵結DTPA數量之分析 5-2-2 未反應的free Gd3+之檢測 5-2-3 Dendrimer-DTPA螯合Gd3+數量之分析 5-2-4 樹枝狀高分子顯影劑Relaxivity之比較 5-3 Dendrimer鍵結葉酸/MTX量之分析 5-3-1 Dendrimer鍵結葉酸 5-3-2 Dendrimer鍵結MTX 5-4 多功能樹枝狀高分子載體之製備 5-4-1 合成Dendrimer-FA-(DTPA-Gd) 5-4-2 合成Dendrimer-FA-MTX 58 5-5 體外細胞實驗(in vitro biological test) 5-5-1 細胞毒性測試(cytotoxicity) 5-5-2 細胞磁振造影(cell MRI) 第六章 結論 第七章 參考文獻

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