分子識別 （例如：抗原−抗體，DNA−DNA和鏈黴親合素−生物素）是用於辨識目標分析物與訊號放大的現代生物分析之方法之一。此方法不適用於偵測金屬離子、陰離子與小分子 (如H2O2及O2−)，因為這些分子太小而不能有效地結合到大分子上。因此，我們設計鏈黴親合素與生物素開關控制的結合探針 (SBIO probes)，藉由探針辨識目標分子，透過酵素或螢光染料綴合的鏈黴親合素反應，對氟離子偵測訊號進行放大，並應用於網版印刷電極與微陣列之檢測技術。與傳統的分析方法相比，籠閉生物素探針展現了更低的背景值，且不受複雜環境中其他分子的干擾，甚至能運用在不同種類的檢測技術上。我們相信此新型檢測分析方法有助於醫學上的診斷與基礎生物學研究。

Molecular recognition (e.g. antigen-antibody, DNA-DNA and streptavidin-biotin) is fundamental in modern bioanalytical methods for target recognition and signal amplification. However, this approach is not applicable to metals, anions and small reactive molecules (e.g. H2O2 and O2−) as these molecules are too small to bind effectively to the macromolecule. Therefore, we demonstrate a general “OFF-ON” molecular recognition approach based on streptavidin-biotin controlled binding probes (SBIO probes) for enzyme-catalyzed signal amplified detection of fluoride (F−) with electrochemical detection techniques. This versatile approach can also be applied in microarray by using streptavidin conjugated with fluorescent dye reporters for target detection. As compared to the conventional analytical methods, the new strategy has several advantages, such as minimal background in its “OFF” state, multiple signal amplification steps and an unlimited selection of detection techniques. We believe that this SBIO probe strategy will be useful for a wide range of applications, such as in medical diagnosis and basic biological research, where highly specific signal enhancement is required.

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