生物醫學工程與醫學研究結腸上皮癌細胞（Caco-2），仍利用傳統培養皿進行細胞培養，培養皿與人體真實結構上差異極大，人體腸道組織是由小腸絨毛與大腸腺窩所組成，皆為三維立體結構，但培養皿為二維平面非三維的立體結構。近期在許多的醫學研究報告中指出二維與三維組織會有明顯的差異，如：細胞與細胞密度、藥物吸收與釋放速率、細胞與細胞之間的相互影響力、細胞生長速度、平面與重直壁細胞的攀附力...等等。因此，本論文的研究重點著重於製作仿小腸絨毛結構支架與大腸腺窩結構支架（Scaffold），提供一個適合腸細胞生長的環境，藉由這個仿生結構可以在體外進行腸道組織的研究，此結構對於生醫與醫學上會有很大的幫助。
本實驗利用微機電製程，以背後曝光技術和SU-8光阻，並參考光學模擬，製作出具有傾斜角度的微針陣列，利用PDMS翻膜得到高分子微針陣列來仿造大腸腺窩與小腸絨毛生理結構，成功製作出高度為350 μm、結構底部半徑為150 μm的小腸絨毛結構；高度為500 μm、結構底部半徑為100 μm的大腸腺窩結構。並在此三維結構在上種植Caco-2細胞，進行細胞培養。
細胞培養的部分已經順利在仿生結構上培養Caco-2細胞形成組織，從共軛焦顯微鏡（Confocal microscope）與電子顯微鏡SEM（Scanning electron microscope）拍攝的結果，Caco-2細胞成功生長於絨毛與腺窩結構組織上。在單位1平方公分面積中，生長在三維腸道結構上的細胞數量約為4.43×105/ ml，在平面的細胞數約為 1.78 ×105 / ml，在立體微結構上細胞生長的數目比平面細胞生長的數量增加了2.5倍；在Caco-2細胞培養兩週後，待細胞完整包覆本三維立體結構，細胞有良好的生長，並在細胞與細胞間形成Tight-junction。
本技術可以用來製作人體內真實小腸絨毛與大腸腺窩組織支架，並進行細胞培養研究，此結構提供了腸細胞三維生長環境，大大改善細胞生長環境。

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