植物非專一性脂質運輸蛋白(nsLTPs)是一種小分子量的鹼性蛋白，其結構是以α-螺旋為主體的結構。依照分子量的不同此一蛋白可分為兩大類，即nsLTP1 (9k Da)和nsLTP2 (7k Da)。相對於nsLTP1，只有稻米和小麥的nsLTP2結構被解出來。因此，為了更加了解nsLTP2的特性，我們利用同源性建模方法和分子嵌合技術來研究nsLTP2的結構、生物物理和生物化學特性。除此之外，植物非專一性脂質運輸蛋白在植物的防禦機制上似乎扮演重要的角色，儘管真正的抗菌機制還不是很清楚。我們在此提出此一蛋白藉由與固醇類分子的結合以促進抗菌的反應發生。為了研究此課題，我們利用了螢光光譜、原二色光譜儀、質譜儀和核磁共振儀來進行研究。同時，我們也利用分子生物學的點突變技術來加以闡明疏水性胺基酸對於此蛋白結構和與固醇類分子結合的影響。最後，由於植物非專一性脂質運輸蛋白具有與不同受質的結合能力以及高穩定度的結構特性，因此此蛋白有潛力成為藥物傳送的載體。我們利用了電腦助藥物篩選的技術來進行篩選，試找出可能與此一蛋白結合的藥物，並結合螢光光譜的方法加以驗證。如此，可進一步提高此一蛋白質的附加價值。

Plant nonspecific lipid transfer proteins (nsLTPs) are small basic proteins which are characterized by their ability to transfer lipids between membranes in vitro. Based on the molecular weight, nsLTPs are classified into two subfamilies, nsLTP1 (9 kDa) and nsLTP2 (7 kDa). In contrast with nsLTP1, only rice and wheat nsLTP2 structures have been determined in this subfamily. In order to realize nsLTP2s, comparative modeling and molecular docking were used to study their structural, biophysical and biochemical properties. Besides, nsLTPs seem to play an important role in plant defense although the exact mechanism for antimicrobial effects is still unclear. We purpose that nsLTPs may associate with a sterol molecule to trigger the plant defense. This hypothesis was verified by using various spectroscopic techniques like fluorescence, circular dichroism, mass spectrometry and NMR. We also used site-directed mutagenesis to investigate the influence of hydrophobic residues on sterol binding and protein stability. Finally, the wide binding properties allow nsLTPs to be a potential drug carrier. Therefore, we used a computer-based high throughput screening (HTS) method to screen drug library to identify the possible binding drugs, thus increasing the added value for nsLTPs in drug delivery systems.

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