醛類在生活中經常可見，其高反應性的醛基使得它在有機合成等領域具廣泛應用，但同時也容易造成對人體的危害，雙醛則是具備二個醛基的化合物，使其經常作為交聯劑及消毒劑使用。醛的測量一般使用2,4-二硝基苯肼衍生法搭配HPLC-UV/vis進行分析，然而此方法在測量雙醛時，會因為多種衍生產物而有所限制，造成定量上的困難。本研究使用戊醛、鄰苯二甲醛、戊二醛、鄰硝基苯甲醛、2-羧基苯甲醛及2,3-萘二甲醛，在不同溶劑下進行衍生反應，測量衍生時間改變的影響以及計算反應速率常數。發現戊二醛的檢量線斜率為戊醛的2.3倍，且芳香醛衍生物的最大吸收波長會相較於直鏈碳醛從360nm紅移至390nm附近，衍生反應速率在乙腈溶劑中明顯較去離子水溶劑中快速，反應速率常數在乙腈溶劑中分別為1.4430 min-1 (對磷苯二甲醛)、0.5104 min-1 (戊醛)；在去離子水溶劑中則為0.0140 min-1 (對磷苯二甲醛)、0.2885 min-1 (戊醛)。

Aldehydes are commonly chemical compounds in the living environment and they are widely applicable in organic synthesis and other fields because of their high reactivity. However, they are also hazardous to human health. Dialdehydes, compounds with two aldehyde groups, are often as cross-linking agents and disinfectants. The measurement of aldehydes employs typically 2,4-dinitrophenylhydrazine (DNPH) derivatization method coupled with HPLC-UV/vis. However, this method faces limitations in quantification when measuring dialdehydes due to the formation of mono/dime-derivative products. In this study, derivatization reactions were conducted with pentanal, o-phthalaldehyde (OPA), glutaraldehyde (GA), 2-nitrobenzaldehyde (2-NB), 2-carboxybenzaldehyde (2-CBA), and naphthalene-2,3-dicarboxaldehyde (NDA) in different solvents to investigate the effect of derivatization time and to calculate the reaction rate constants. It was found that the absorbance values of glutaraldehyde derivatives were approximately 2.3 times higher than those of pentanal derivatives. Moreover, the maximum absorption wavelength of aromatic aldehyde derivatives shifted from 360nm to 390nm compared to straight-chain aldehyde derivatives. The reaction rate constants of OPA and pentanal are 1.4431 min-1 and 0.5104 min-1 in acetonitrile (ACN) and 0.0140 min-1 and 0.2885 min-1 in water solvent, respectively.

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林奕良, 碩士論文. 國立清華大學, 新竹市, 2005.
葉雲臺, 碩士論文. 國立清華大學, 新竹市, 2009.