本計畫書旨在製備不同官能基修飾之葡聚醣包覆磁性氧化鐵奈米粒子，後欲將其及應用於生物醫學領域。由於葡聚醣於生物醫學上不會對細胞產生生物毒性，因此不會受到細胞排斥，因此葡聚醣適合應用於生物醫學領域。
實驗上先以共沉法製備無修飾之磁性奈米粒子，並以物理性研磨的方式使葡聚醣能夠均勻包覆在磁性奈米粒子表面，再採用不同化學反應修飾不同種官能基(包含：氨基(-NH2)、羧基(-COOH)以及羥基(-OH))到葡聚醣包覆之磁性奈米粒子表面，使磁性奈米粒子分別具有的官能基。
材料分析部分，使用超導量子干涉磁量儀(Superconducting quantum interference device，SQUID)、穿透式電子顯微鏡(Transmission electron microscope， TEM)及霍氏轉換紅外光譜儀 (Fourier transform infrared spectroscopy，FTIR)分別進行磁性奈米粒子的磁性、大小及表面修飾官能基的鑑定。藉由熱重分析儀(Thermogravimetric analysis，TGA)確認磁性奈米顆粒的葡聚醣包覆率以及官能基修飾量，並以熱示差描熱卡量計(Differential scanning calorimetry，DSC)獲知相變化的焓變化量。
此外，將實驗室上屆畢業生黃俊榮學長研發之3-(3.4-羥基苯基)丙酸(3-(3,4-Dihydroxy)hydrocinnamic acid，DHCA)包覆四氧化三鐵進行抗癌藥物-帝盟多(Temozolomide，TMZ)修飾反應，利用DHCA提供之羧基官能基與TMZ鍵結，獲得抗癌藥物包覆之四氧化三鐵。再利用紫外光-可見光光譜儀(UV-vis spectrometer)檢測TMZ是否存在於顆粒表面，以證明藥物是否修飾成功。

　　Dextran molecules with low bio-toxicity is beneficial for biomedical fields. Dextran-coated superparamagnetic iron oxide nanoparticles (SPIONs) can have broader applications if modified with different functional groups. Therefore, the aim of the thesis is to synthesiz iron oxide nanoparticles with functional groups for biomedical appications.
　　Iron oxide nanoparticles (Fe3O4) were prepared by co-precipitation method and mixed with dextran solution under ball milling conditions to minimum particles size and assist dextran capping on particle surface. Different functional groups including amino (-NH2), carboxyl (-COOH) and hydroxyl groups (-OH) were then modified to dextran- Fe3O4 utilizing different chemical reactions. Superconducting quantum interference device (SQUID) and transmission electron microscope (TEM) were used to characterize and prove the superparamagnetism, size and shape. Fourier transform infrared spectroscopy (FTIR) analyzed the presence of functional groups. Thermogravimetric analysis (TGA) confirmed the mass percent of dextran and functional groups on iron oxide nanoparticles and phase transition enthalpy was determined by differential scanning calorimetry (DSC).
　　Lastly, anti-cancer drugs, Temozolomide (TMZ), was modified on to the carboxyl groups of 3-(3,4-Dihydroxy)hydrocinnamic acid (DHCA) coated Fe3O4. UV-vis spectrometer was used to analyze the presence of TMZ on the particles and prove the successful modification.

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