纖維母細胞生長因子(FGF)在體內扮演許多重要的角色，諸如促進細胞增生、細胞分化、傷口癒合等，甚至近幾年發現它和腫瘤的形成也有關係。由於FGF和肝素(heparin)結合時，不僅三級結構會變得比較穩定，甚至還會增強FGF的生物活性，因此在本文中，我們探討一個類肝素分子(heparin-like compound)-Sucrose Octasulfate(SOS)對於FGF穩定度的影響，發現使用SOS可以保護FGF的三級結構受到酸鹼值及尿素的破壞，同時在莫耳鍵結比例為1時，達到飽和。我們也針對FGF在開散路徑中平衡中間物的探索及性質有一些研究，藉由螢光實驗、圓二色光學實驗及ANS尿素開散實驗，發現平衡中間物存在於pH=5.0、2.4 M 尿素濃度下，當我們更進一步使用核磁共振技術去觀測平衡中間物的結構時，發現雖然HSQC光譜相較於自然態的光譜，沒有太大的差異；但是經由氫氘交換後，有許多的殘基消失，分析得知在平衡中間物態時，大部份維持三級結構的作用力消失，而同時也只剩下50%的二級結構存在。

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