單克隆細胞的抗體檢測主要用於選擇雜交瘤細胞和轉染的中國倉鼠卵巢細胞 (Chinese hamster ovary cell ; CHO cell)。經過篩選的雜交瘤細胞和轉染的中國倉鼠卵巢細胞可產生單株抗體以提供傳染病，腫瘤，關節炎，免疫疾病和代謝疾病的治療。然而，在雜交瘤細胞形成和中國倉鼠卵巢細胞轉染期間，會有不產抗體的細胞以及產抗體的細胞產出，這導致其難以獲得特異性抗體並且降低其有效治療，所以篩選單克隆細胞在製備治療用抗體是重要的。一般篩選方法使用有限稀釋或半固體培養基技術來分離成單細胞，並且在培養幾週後，用酵素結合免疫吸附分析法(Enzyme-linked immunosorbent assay ; ELISA)檢測細胞培養的培養液，進而篩選會產抗體的細胞，但是此種篩選方式需要付出高成本的時間和勞動力。為了減少篩選細胞所需的成本，本研究將以提高篩選單克隆細胞的效率開發一種微流體晶片。本晶片上有三種結構：雙孔，柱子和收集孔。雙孔結構提供抓取單細胞及培養能力；柱子結構藉由不互溶的液體，產生分離的液滴並建立獨立檢測空間；收集孔結構是為了均勻收集作為探測器的玻璃微泡，以提供一致的檢測標準和提升螢光訊號。根據上述功能，本裝置被視為依據抗體檢測篩選單克隆細胞的快速平台並且在未來有機會取代傳統的方法。

Antibody detection of the monoclonal cell is mainly used in the selection of hybridoma and transfected CHO cell (Chinese hamster ovary cell). The screened hybridoma and transfected CHO cells can produce monoclonal antibody (mAbs) to provide treatment of infectious diseases, tumors, immune diseases, and metabolic diseases. However, during hybridoma formation and CHO cell transfection, there are non-antibody-producing cells and antibody-producing cells, which results in difficulty in obtaining specific antibodies and the reduction of their therapeutic validity. Therefore, screening monoclonal cells is important in the antibody-based therapeutics. The conventional screening method uses limiting dilution or semi-solid medium technology to isolate transfected cells into single cells and after culturing for several weeks, medium drawn from cell culture is detected by ELISA (Enzyme-linked immunosorbent assay) to select monoclonal cells. This method requires a significant amount of time and labor, which causes monoclonal antibodies to be expensive. To reduce the cost of screening, this research aims to develop a microfluidic device to increase the screening efficiency of monoclonal cells. The device has three structures: dual wells, pillars, and collection well. Dual-well structure provides single cell capture and culture function. Pillars can create isolated droplets and build independent detection space when an immiscible liquid flows through this structure. Collection well structure is to evenly collect glass microbubbles as detectors to supply the same benchmark of detection and enhance the fluorescence signal. According to the above functions of the device, the device is considered as a platform to screen monoclonal cells based on antibody detection and it will have a chance to replace the conventional method in the future.

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