本實驗利用已廣泛運用於經皮給藥的磷脂質(phospholipids)，應用於包覆目前唯一能阻斷黑色素轉移的美白成分水溶性維他命B3 (niacinamide)並添加不同的經皮吸收增加劑(penetration enhancer)，藉著Franz-type擴散槽進行皮膚穿透實驗，以得知不同配方的穿透效果。
實驗結果發現含有20%酒精的配方Soybean phosphotidylcholine -sodium cholate (SPC-sc)及SPC-vitE (tocopherol)，能有效地增加niacinamide的穿透係數；含有20%酒精配方SPC-vitE及不含酒精配方的SPC-oleic acid (SPC-oa)， niacinamide在皮膚裡的累積量最多，以上三種配方能使niacinamide穿透過角質層或是留在皮膚裡，與微脂粒粒徑及包覆效率無絕對關係，並提出可能穿透機制為，排列較為不緊密的微脂粒脂雙層，使角質層脂質膨潤，增加其流動性，進而可能發生膜融合(fusion)現象，以增加活性分子進入皮膚機會；另外，脂質配方及酒精成份存在著某種協合作用，使活性分子穿透或是累積在皮膚裡的量為之提升。

The influence of phospholipids based formulations on the in vitro percutaneous penetration of niacinamide was examined using abdominal skin of Wistar mouse mounted on Franz-type diffusion chamber. Transepidermal permeation through excised mouse skin and skin deposition of niacinamide from different formulations were assessed and compared. Liposome in water solution did not enhance the skin permeability of niacinamide, but when ethanol (20% v/v) was present in the donor with SPC-vitE or SPC-sc, permeability of niacinamide was increased. Liposome size and entrapment efficiency were determined. The encapsulation efficiency of niacinamide was found to be very low, however, the amount of absorbed niacinamide was nearly independent of the encapsulation and the vesicle size. Based on the results obtained, liposome components in solution have additive effect with a possible synergism in some cases.

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