隨著檢驗技術的進步，快速、低錯誤率和易操作性成為了檢驗技術開發的核心理念，相較以往耗時的儀器檢測或是需要專業人員操作的生化測試，成本低廉且容易使用的生物探針成為了現在發展的主流。
在本論文中，我們開發出新式的可調控生物素探針，利用生物素(Biotin)和鏈黴親合素(Streptavidin)之間可變親和力的結合，以達到高專一性的分析物檢測，並將其應用在螢光訊號增強以及側向流量層析的檢測。
在論文的第一部分，我們結合可調控生物素探針和環境敏感型的螢光分子SBD (4-sulfamoyl-7-amino benzoxadiazole)，以進行過氧化氫(H2O2)的定性和定量，並將其用來檢驗胎牛血清(Fetal Bovine Sera)中葡萄糖的含量。
在第二部分的論文，我們將可調控生物素探針應用在競爭型的金奈米粒子側向流量層析檢測法(Competitive Lateral Flow Assay)，解決了競爭型側向流量層析檢測無法以正向訊號呈現的基礎問題。我們以磺胺類小分子的檢測爲例，當環境中沒有磺胺類小分子時，因生物素和鏈黴親和素之間的立體障礙而無法鍵結，在測試線上沒有訊號產生。在磺胺類小分子的存在下，生物素和鏈黴親和素之間沒有有立體障礙，因此可以順利結合在測試線上呈現訊號。我們成功開發出第一個產生金奈米粒子正向訊號的小分子檢測側向流量層析檢測法。

Rapid, high reproducibility, and simple operation are the important factors in the development of advanced diagnostic techniques. In contrast to the time-consuming biochemical testing that required expensive instruments and skilled professionals, cost-effective and user-friendly chemical probes have emerged as the mainstream approach for chemical analysis.
In this thesis, we develop a novel affinity-switchable biotin-streptavidin binding approach for the signal amplified fluorescence sensing and lateral flow assay of small molecules. In the first part of the thesis, an affinity switchable biotin probe conjugated with an environment-sensitive SBD fluorophore (4-sulfamoyl-7-amino benzoxadiazole) was developed for the fluorescent signal amplified detection of hydrogen peroxide. We also showed that this affinity switchable biotin probe can also be used in blood serum to quantify the glucose concentration.
In the second part, affinity-switchable biotin probe was applied on competitive lateral flow assay (LFA) for the SIGNAL-ON detection of small organic molecules. In the absence of a small molecule, a large steric hindrance was generated which prevents the effective binding of the biotin and streptavidin protein. Therefore, a test line was not observed on the LFA membrane. In the presence of a small molecule target, this steric hindrance is removed, thereby exposing the biotin for streptavidin binding to produce a test line. We demonstrated in this thesis that small molecule sulfonamide drug can be selectively detected using this simple and modular affinity-switchable lateral flow assay (ASLFA).

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