由於先前學者利用S-layer自我形成二維結構的能力並將之鋪排在固相表面上用來作為待測物之儲存槽。而本研究是在晶片製程過程中，利用複合性生物材料結合螢光在晶圓表面上自我形成反應平台。本實驗室採用含有自我組裝能力S-layer的生物材料(稱為SOM)來當作主要的基材。其材料是在高鹽濃度下培養耐輻射奇異球菌生長直到靜止期時所產生的SOM，發現此SOM含有與生物膜的組成具有一定的關連性。
為了避免需要額外添加螢光染劑造成背景值增加，故添加可發散出紅光的銀顆粒來修飾SOM(複合性的SOM)。我們發現在晶圓表面上聚集成微米大小的圓點，並使之塗佈在矽晶圓表面修飾且大小均一。當合成的複合物散佈在晶圓表面上會形成單一的螢光圓點，並進一步瞭解螢光強度與SOM之間的關係，經由統計發現其SOM對於螢光的效率和光漂白現象影響不大。再者，生物晶片需要很高的靈敏度，此來自於反應區域的多寡，我們也發現發現此複合物會產生大約大小為2微米，並經過計算在每平方0.7x0.7公分的晶圓總共大約有2.75x105 個獨立的圓點。總結來說，這種利用具有功能性的生物材料可以自我組成並形成2μm單一獨立大小反應的圓點。

A biologist took advantage of S-layer formed planner structure and applied to loading capacity on solid support. In this thesis, we create fluorescent spots with biomaterial fabricates platform on wafer. We adopt the biomaterial (SOM) produced from bacterial, which including S-layer protein and its composition is correlation with bacterial membrane.
To avoid additional fluorescent dye produced background on wafer, the biomaterial mixed with fluorescent silver particle. We use confocol microscope to observe the micro-sized fluorescent complex (complex SOM) composed of SOM and silver particle adhere on the wafer surface. For studying on fluorescent light with complex SOM , SOM couldn’t affect on efficiency and photobleaching . when its spot size is 2μm, there are more than 2.75x105 micro-sized spot individually on the 0.7x 0.7 cm2 wafer. In conclusion, this approach can be self-assemble, disperse the functional SOM, set itself independently like array and form 2μm size on the wafer .

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