摘要
本篇研究中，我們利用LA-ICP-MS結合MATLAB建立元素分佈的生物影像技術，而這項技術用於提供二維及三維度的元素分佈影像，並且可計算出特定區域的平均訊號強度。我們也利用此技術分析一片經過MFH治療且含有Fe修飾Gd之奈米粒子的腫瘤切片，將分析的結果處理後發現，Fe元素的分佈與Gd元素的分佈有很高的相依性，這代表Fe修飾Gd之奈米粒子在進入腫瘤後並沒有被分解；而在生物體中的必要元素如C、P、S的元素分佈圖則顯示，在其中有一塊訊號強度較低的空洞區域，而這塊區域與接受MFH治療區相同。因此，LA-ICP-MS結合MATLAB技術不僅可以提供生物組織中必要元素的分佈情況，未來還可用於提供癌症治療的資訊。
Abstract
In this study, we used laser ablation inductively coupled mass spectrometry (LA-ICP-MS) to establish the bioimage technique of multi-element distribution with the assistance of matrix laboratory software (MATLAB). This technique is applied to provide the two- and three-dimensional image and to calculate the mean intensities of particular regions. We also employed this technique to produce the elemental distribution of a tumor slice, which contained Gd-doped iron nanoparticles (IONPs) during the treatment of magnetic fluid hyperthermia (MFH). The results of data processing revealed a high correlation between the distribution of Fe atoms and Gd atoms and proved that the IONPs entering into the tumor didn’t decompose. The distribution of essential elements such as C, P, and S in an organism had an empty region where the MFH treatment had been. Consequently, the LA-ICP-MS technique related to MATLAB can not only produce the spatial distribution of essential elements in tissue but also provide the information of cancer treatment in the tumor in the future.

In this study, we used laser ablation inductively coupled mass spectrometry (LA-ICP-MS) to establish the bioimage technique of multi-element distribution with the assistance of matrix laboratory software (MATLAB). This technique is applied to provide the two- and three-dimensional image and to calculate the mean intensities of particular regions. We also employed this technique to produce the elemental distribution of a tumor slice, which contained Gd-doped iron nanoparticles (IONPs) during the treatment of magnetic fluid hyperthermia (MFH). The results of data processing revealed a high correlation between the distribution of Fe atoms and Gd atoms and proved that the IONPs entering into the tumor didn’t decompose. The distribution of essential elements such as C, P, and S in an organism had an empty region where the MFH treatment had been. Consequently, the LA-ICP-MS technique related to MATLAB can not only produce the spatial distribution of essential elements in tissue but also provide the information of cancer treatment in the tumor in the future. Keywords：Laser ablation inductively coupled mass spectrometry; magnetic fluid hyperthermia; elemental image

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