研究生: |
楊智舜 Yang, Chi-Shun |
---|---|
論文名稱: |
昆蟲自動化計數與分類控制平台 ─ 以果蠅為例 Automated Counting and Classification of Insect Control System – in Drosophila |
指導教授: |
陳榮順
Chen, Rongshun |
口試委員: |
黃安橋
蔡宏營 |
學位類別: |
碩士 Master |
系所名稱: |
工學院 - 動力機械工程學系 Department of Power Mechanical Engineering |
論文出版年: | 2011 |
畢業學年度: | 99 |
語文別: | 中文 |
論文頁數: | 68 |
中文關鍵詞: | 昆蟲刺激 、行為監測平台 、基因工程 、昆蟲計數 、LabVIEW控制 |
相關次數: | 點閱:1 下載:0 |
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黑腹果蠅具備完整的基因序列分析,且基因改造技術與行為模式研究成熟,生命週期短、培養容易、飼養成本低、與人類基因相似度更高達60 %相同,故廣泛作為研究人類基因之跳板。由於果蠅實驗需要大量實驗數據進行生物統計,而傳統上繁複的果蠅分類與計數工作皆以人工方式完成,不僅相當耗費人力與時間,也易因人工疏失產生誤差,且每次進行的實驗數目亦有限,無法有效率得到大量實驗數據。為此,本研究提出以果蠅為例之昆蟲自動化計數與分類控制平台,期望能以自動化方式實現大規模進行實驗之可能。
本研究設計果蠅輸送計數系統與處女果蠅性別辨識控制載台,藉由自行設計之光電傳感器模組與LabVIEW人機介面,實現對果蠅計數之目標,以一百隻果蠅為例,利用果蠅計數系統進行自動計數與人工計數比較後所得之準確度達96.8 %。處女果蠅性別辨識控制載台,則與機械視覺辨識系統結合,以果蠅腹部特徵點辨識性別,利用果蠅之天生習性驅使與抽氣輔助方式對剛羽化之果蠅進行蒐集控制,順利實現辨識未交配果蠅之公母性別並同時進行蒐集。實際應用於果蠅實驗中,即可大量節省珍貴之實驗人力與時間。
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