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研究生: 黃建榮
Huang, Chien-Jung
論文名稱: 果蠅睡眠監控平台與噪音干擾實驗
Drosophila Sleep Monitor Platform and Noise Disturbance Experiment
指導教授: 賀陳弘
Hocheng, Hong
口試委員: 洪景華
林士傑
學位類別: 碩士
Master
系所名稱: 工學院 - 動力機械工程學系
Department of Power Mechanical Engineering
論文出版年: 2011
畢業學年度: 99
語文別: 中文
論文頁數: 76
中文關鍵詞: 果蠅光源刺激源氣味刺激源多刺激源模組平台音源刺激風源刺激源睡眠干擾實驗
外文關鍵詞: Drosophila, multiple simulation modules, light stimulation, odor stimulation, sound stimulation, wind stimulation, sleep disturbance experiment
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  • 目前在果蠅腦科學研究上,多以分散式的感測刺激儀器輔以人工方式進行行為資訊的擷取,且由Trikinetic公司販售的果蠅睡眠平台在果蠅睡眠實驗上,仍有些許缺陷。因此本論文設計一新穎的多刺激源模組睡眠監控平台,輔以自動化程式控制,並讓刺激源模組化,能集中在同一平台上進行多種刺激實驗,也可依實驗者需求同時或分開進行控制刺激。本論文也利用此平台,使用音源做為主要刺激源,針對果蠅的睡眠在夜晚播放以期達到干擾效果,希望能改以非接觸的方式來降低果蠅可能因外力造成的傷害。實驗所使用的音源為「脈衝式」的白噪音,於夜晚進行隨機地干擾並以紅外線攝影機錄下晝夜果蠅活動情形,最後由影像分析做後處理。希望此次創新的多刺激源睡眠平台與音源干擾實驗,可以在未來果蠅聽力研究與睡眠研究方面,提供全新的研究模型,並藉著多刺激源模組功能,讓未來科學家可以用更多變化的刺激方式做更深入地研究。


    第 1 章 緒論 1 1.1 背景 1 1.2 研究動機 5 第 2 章 文獻回顧 7 2.1 嗅覺與果蠅 7 2.2 光與果蠅 14 2.3 聽覺與果蠅 18 2.4 風與果蠅 21 2.5 其他刺激源(溫度、濕度) 22 第 3 章 睡眠平台與多刺激源模組設計 27 3.1 現有睡眠平台 27 3.2 睡眠平台設計 28 3.3 多刺激源模組設計 29 3.3.1 光源模組 30 3.3.2 嗅覺模組 33 3.3.3 風源模組 34 3.3.4 自動監控程式 37 3.3.5 多重刺激源設計整合 39 第 4 章 噪音干擾實驗方法 41 4.1 目前果蠅睡眠實驗研究 41 4.2 睡眠干擾實驗 45 4.2.1 實驗目的 45 4.2.2 音源設計 47 4.2.3 實驗方法 51 第 5 章 實驗結果與討論 54 5.1 求偶歌與果蠅睡眠影響 54 5.2 脈衝式白噪音睡眠干擾結果 58 5.3 實驗結果討論 60 5.3.1 血清素與果蠅睡眠 60 5.3.2 血清素受體與果蠅睡眠 61 5.3.3 內分泌物質與果蠅睡眠 62 5.3.4 其他可能原因 63 第 6 章 結論與未來工作 64 第 1 章 緒論 1 1.1 背景 1 1.2 研究動機 5 第 2 章 文獻回顧 7 2.1 嗅覺與果蠅 7 2.2 光與果蠅 14 2.3 聽覺與果蠅 18 2.4 風與果蠅 21 2.5 其他刺激源(溫度、濕度) 22 第 3 章 睡眠平台與多刺激源模組設計 27 3.1 現有睡眠平台 27 3.2 睡眠平台設計 28 3.3 多刺激源模組設計 29 3.3.1 光源模組 30 3.3.2 嗅覺模組 33 3.3.3 風源模組 34 3.3.4 自動監控程式 37 3.3.5 多重刺激源設計整合 39 第 4 章 噪音干擾實驗方法 41 4.1 目前果蠅睡眠實驗研究 41 4.2 睡眠干擾實驗 45 4.2.1 實驗目的 45 4.2.2 音源設計 47 4.2.3 實驗方法 51 第 5 章 實驗結果與討論 54 5.1 求偶歌與果蠅睡眠影響 54 5.2 脈衝式白噪音睡眠干擾結果 58 5.3 實驗結果討論 60 5.3.1 血清素與果蠅睡眠 60 5.3.2 血清素受體與果蠅睡眠 61 5.3.3 內分泌物質與果蠅睡眠 62 5.3.4 其他可能原因 63 第 6 章 結論與未來工作 64 6.1 結論 64 6.2 未來工作 65 第 7 章 參考文獻 66 附錄A 70

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